Tim Overton

Accepting PhD Students

PhD projects

We are interested in using information about the molecular microbiology and physiology of bacteria to develop processes. We focus on three main areas:

- Development of bioprocesses that generate high-value products (for example recombinant protein biopharmaceuticals, polymers, small molecules);
- Understanding biofilms and they ways in which their structure and function are regulated and might be modulated; and
- Developing processes that are designed to eliminate contaminating bacteria from product streams (for example foods and waste streams).

Research activity per year

65 Article
3 Chapter
3 Review article
2 Abstract
More

1 - 50 out of 77 results

2024
A scalable biomanufacturing platform for bacterial magnetosomes
Fernández-Castané, A., Li, H., Ebeler, M., Franzreb, M., Overton, T. & Thomas, O., Mar 2024, In: Food and Bioproducts Processing. 144, p. 110-122 13 p., FBP-D-23-00899.
Research output: Contribution to journal › Article › peer-review

Open Access
File
53 Downloads (Pure)
Efflux pumps mediate changes to fundamental bacterial physiology via membrane potential
Whittle, E., Orababa, O., Osgerby, A., Siasat, P., Element, S., Blair, J. & Overton, T., 9 Sept 2024, (E-pub ahead of print) In: mBio. e02370-24.
Research output: Contribution to journal › Article › peer-review

Open Access
File
37 Downloads (Pure)
Integrated system for temperature-controlled fast protein liquid chromatography. IV. Continuous ‘one-column’ ‘low-salt’ hydrophobic interaction chromatography
Brean, A., Overton, T., Bracewell, D., Franzreb, M. & Thomas, O., 30 Aug 2024, In: Journal of Chromatography A. 1731, 14 p., 465212.
Research output: Contribution to journal › Article › peer-review

Open Access
File
18 Downloads (Pure)
Nanovibrational Stimulation of Escherichia coli Mitigates Surface Adhesion by Altering Cell Membrane Potential
Bazzoli, D. G., Mahmoodi, N., Verrill, T.-A., Overton, T. W. & Mendes, P. M., 22 Oct 2024, (E-pub ahead of print) In: ACS Nano.
Research output: Contribution to journal › Article › peer-review

Open Access
File
15 Downloads (Pure)
2023
Approaches for High-throughput Quantification of Periplasmic Recombinant Proteins
Osgerby, A. & Overton, T., 25 Nov 2023, In: New Biotechnology. 77, p. 149-160 12 p.
Research output: Contribution to journal › Review article › peer-review

Open Access
File
148 Downloads (Pure)
Efflux pumps mediate changes to fundamental bacterial physiology via membrane potential
Whittle, E., Orababa, O., Element, S., Blair, J. & Overton, T., 3 Apr 2023, bioRxiv.
Research output: Working paper/Preprint › Preprint
Impact of Environmental Stresses on the Antibacterial Activity of Graphene Oxide (GO) Nanoparticles against P. putida Biofilms
Fallatah, H., Overton, T., Ali-Boucetta, H. & Gkatzionis, K., 28 Feb 2023, In: Microorganisms. 11, 3, 13 p., 609.
Research output: Contribution to journal › Article › peer-review

Open Access
File
74 Downloads (Pure)
Optimisation of recombinant TNFα production in Escherichia coli using GFP fusions and flow cytometry
Zulkifly, A., Selas Castineiras, T. & Overton, T., 2 Aug 2023, In: Frontiers in Bioengineering and Biotechnology. 11, 12 p., 1171823.
Research output: Contribution to journal › Article › peer-review

Open Access
File
111 Downloads (Pure)
Plasma activation and its nanoconfinement effects boost surface anti-biofouling performance
Mahmoodi, N., Bazzoli, D., Overton, T. & Mendes, P., 23 Feb 2023, In: Advanced Materials Interfaces. 10, 6, 10 p., 2202087.
Research output: Contribution to journal › Article › peer-review

Open Access
File
42 Downloads (Pure)
2022
Inexpensive protein overexpression driven by the NarL transcription activator protein
Hothersall, J., Lai, S., Zhang, N., Godfrey, R., Ruanto, P., Bischoff, S., Robinson, C., Overton, T., Busby, S. & Browning, D., 13 May 2022, In: Biotechnology and Bioengineering. 119, 6, p. 1614-1623 10 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
130 Downloads (Pure)
New vectors for urea-inducible recombinant protein production
Hothersall, J., Osgerby, A., Godfrey, R., Overton, T., Busby, S. & Browning, D., 25 Dec 2022, In: New Biotechnology. 72, p. 89-96 8 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
98 Downloads (Pure)
Polymer-induced biofilms for enhanced biocatalysis
Adoni, P., Romanyuk, A., Overton, T. & Fernandez-Trillo, F., 24 Mar 2022, ChemRxiv, (ChemRxiv).
Research output: Working paper/Preprint › Working paper
Polymer-induced biofilms for enhanced biocatalysis
Adoni, P., Romanyuk, A., Overton, T. & Fernandez-Trillo, F., 1 Oct 2022, In: Materials Horizons. 9, 10, p. 2592-2602 11 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
56 Downloads (Pure)
Process Intensification at the expression system level for the production of 1-phosphate aldolase in antibiotic-free E. coli fed-batch cultures
Pasini, M., Fernández-Castané, A., Caminal, G., Overton, T. & Ferrer, P., 6 Jun 2022, In: Journal of Industrial Microbiology and Biotechnology. 49, 4, 11 p., kuac018.
Research output: Contribution to journal › Article › peer-review

Open Access
File
98 Downloads (Pure)
Use of transposon directed insertion-site sequencing to probe the antibacterial mechanism of a model honey on E. coli K-12
Masoura, M., Milner, M., Overton, T., Gkatzionis, K. & Lund, P., 17 Jan 2022, In: Frontiers in Microbiology. 12, 16 p., 803307.
Research output: Contribution to journal › Article › peer-review

Open Access
File
64 Downloads (Pure)
2021
Efflux Impacts Intracellular Accumulation Only in Actively Growing Bacterial Cells
Whittle, E., McNeil, H., Trampari, E., Webber, M., Overton, T. & Blair, J., 12 Oct 2021, In: mBio. 12, 5, e02608-21.
Research output: Contribution to journal › Article › peer-review

Open Access
File
275 Downloads (Pure)
Methods and Products
Fernandez-Trillo, F. (Inventor), Overton, T. (Inventor), Adoni, P. (Inventor) & Huneidi, O. (Inventor), 21 Oct 2021, Patent No. WO2021209765A2, 16 Apr 2021, Priority date 17 Apr 2021, Priority No. GB2005605.7
Research output: Patent
Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations
Fernández-Castané, A., Li, H., Joseph, S., Ebeler, M., Franzreb, M., Bracewell, D., Overton, T. & Thomas, O., May 2021, In: Food and Bioproducts Processing. 127, p. 426-434 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
13 Downloads (Pure)
Pellicle formation by Escherichia coli K-12: role of adhesins and motility
Golub, S. & Overton, T., Apr 2021, In: Journal of Bioscience and Bioengineering. 131, 4, p. 381-389 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
402 Downloads (Pure)
The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli
Hothersall, J., Godfrey, R., Fanitsios, C., Overton, T., Busby, S. & Browning, D., 25 Sept 2021, In: New Biotechnology. 64, p. 1-8 8 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
722 Downloads (Pure)
2020
Nanoparticle Tracking Analysis: A powerful tool for characterizing magnetosome preparations
Fernández-Castané, A., Li, H., Joseph, S., Ebeler, M., Franzreb, M., Bracewell, D., Overton, T. & Thomas, O., 24 Jun 2020, In: bioRkiv.
Research output: Contribution to journal › Article

Open Access
Non-pathogenic Escherichia coli biofilms: effects of growth conditions and surface properties on structure and curli gene expression
Leech, J., Golub, S., Allan, W., Simmons, M. & Overton, T., 28 Mar 2020, In: Archives of Microbiology. 202, 6, p. 1517–1527 11 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
2 Citations (Scopus)

201 Downloads (Pure)
Use of a model to understand the synergies underlying the antibacterial mechanism of H₂O₂-producing honeys
Masoura, M., Passaretti, P., Overton, T., Lund, P. & Gkatzionis, K., 19 Oct 2020, In: Scientific Reports. 10, 1, 17692.
Research output: Contribution to journal › Article › peer-review

Open Access
File
2 Citations (Scopus)

212 Downloads (Pure)
2019
Antibacterial effect of graphene oxide (GO) nano-particles against Pseudomonas putida biofilm of variable age
Fallatah, H., Elhaneid, M., Ali-Boucetta, H., Overton, T., El Kadri, H. & Gkatzionis, K., 1 Aug 2019, In: Environmental Science and Pollution Research. 26, 24, p. 25057–25070 14 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
12 Citations (Scopus)

321 Downloads (Pure)
Flow cytometric analysis of efflux by dye accumulation
Whittle, E. E., Legood, S. W., Alav, I., Dulyayangkul, P., Overton, T. W. & Blair, J. M. A., 4 Oct 2019, In: Frontiers in Microbiology. 10, 10 p., 2319.
Research output: Contribution to journal › Article › peer-review

Open Access
File
6 Citations (Scopus)

187 Downloads (Pure)
Flow cytometry and growth-based analysis of the effects of fruit sanitation on the physiology of Escherichia coli in orange juice
Anvarian, A., Smith, M. & Overton, T., 7 Feb 2019, In: Food Science and Nutrition. 7, 3, p. 1072-1083 12 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
250 Downloads (Pure)
Probing the viability of palladium-challenged bacterial cells using flow cytometry
Omajali, J., Mikheenko, I., Overton, T., Merroun, M. L. & Macaskie, L., 1 Jan 2019, In: Journal of Chemical Technology and Biotechnology. 94, 1, p. 295-301 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
6 Citations (Scopus)

235 Downloads (Pure)
Synthesis of biodegradable polyhydroxyalkanoates from soil bacteria
Kelly, C., Overton, T. & Jenkins, M., 20 Dec 2019, Frontiers in Soil and Environmental Microbiology. Mishra, B. B. & Nayak, S. K. (eds.). 1 ed. Boca Raton: CRC Press
Research output: Chapter in Book/Report/Conference proceeding › Chapter
Use of a Design of Experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions
Kasli, I., Thomas, O. & Overton, T., 7 Jan 2019, In: AMB Express. 9, 5.
Research output: Contribution to journal › Article › peer-review

Open Access
File
9 Citations (Scopus)

287 Downloads (Pure)
2018
A biorefinery approach for fractionation of Miscanthus lignocellulose using subcritical water extraction and a modified organosolv process.
Kubota, A., Kalnins, R. & Overton, T., Apr 2018, In: Biomass and Bioenergy. 111, p. 52-59
Research output: Contribution to journal › Article › peer-review

Open Access
File
10 Citations (Scopus)

501 Downloads (Pure)
Development of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli
Selas Castineiras, T., Williams, S., Hitchcock, A., Cole, J., Smith, D. & Overton, T., 3 May 2018, In: Scientific Reports. 8, 6986.
Research output: Contribution to journal › Article › peer-review

Open Access
File
10 Citations (Scopus)

205 Downloads (Pure)
Development of a simple intensified fermentation strategy for growth of Magnetospirillum gryphiswaldense MSR-1: physiological responses to changing environmental conditions
Fernández-Castané, A., Li, H., Thomas, O. & Overton, T., 25 Nov 2018, In: New Biotechnology. 46, p. 22-30 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
10 Citations (Scopus)

288 Downloads (Pure)
Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing
Gomes, C., Fashina, A., Fernández-Castané, A., Overton, T., Hobley, T. J., Theodosiou, E. & Thomas, O., Jul 2018, In: Journal of Chemical Technology and Biotechnology. 93, 7, p. 1901-1915
Research output: Contribution to journal › Article › peer-review

Open Access
File
6 Citations (Scopus)

259 Downloads (Pure)
Optimising host cell physiology and stress avoidance for the production of recombinant human tumour necrosis factor α in Escherichia coli
Selas Castineiras, T., Williams, S., Hitchcock, A., Cole, J., Smith, D. & Overton, T., 1 Apr 2018, In: Microbiology. 164, p. 440-452
Research output: Contribution to journal › Article › peer-review

Open Access
File
3 Citations (Scopus)

337 Downloads (Pure)
Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli
Anvarian, A., Smith, M. & Overton, T., 16 Oct 2018, In: Food Science and Nutrition. 6, 7, p. 1817-1825
Research output: Contribution to journal › Article › peer-review

Open Access
File
3 Citations (Scopus)

180 Downloads (Pure)
2017
CsrA maximizes expression of the AcrAB multidrug resistance transporter
Ricci, V., Attah, V., Overton, T., Grainger, D. C. & Piddock, L. J. V., 15 Dec 2017, In: Nucleic Acids Research. 45, 22, p. 12798–12807
Research output: Contribution to journal › Article › peer-review

Open Access
File
8 Citations (Scopus)

198 Downloads (Pure)
Do oil-in-water (o/w) nano-emulsions have an effect on survival and growth of bacteria?
Gkatzionis, K., El Kadri, H., Devanthi, P. V. & Overton, T., 6 Sept 2017, (E-pub ahead of print) In: Food Research International.
Research output: Contribution to journal › Article › peer-review

Open Access
File
7 Citations (Scopus)

417 Downloads (Pure)
Flow cytometry as a rapid analytical tool to determine physiological responses to changing O₂ and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1
Fernández-Castané, A., Li, H., Thomas, O. & Overton, T., 13 Oct 2017, In: Scientific Reports. 7, 13118.
Research output: Contribution to journal › Article › peer-review

Open Access
File
8 Citations (Scopus)

169 Downloads (Pure)
2016
Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
Vizcaino Caston, I., Kelly, C., Fitzgerald, A., Leeke, G., Jenkins, M. & Overton, T., 31 Jan 2016, In: Journal of Bioscience and Bioengineering. 121, 1, p. 101-104
Research output: Contribution to journal › Article › peer-review

Open Access
File
7 Citations (Scopus)

745 Downloads (Pure)
Modulating the release of Escherichia coli in double W1/O/W2 emulsion globules under hypo-osmotic pressure
El Kadri, H., Gun, R., Overton, T., Bakalis, S. & Gkatzionis, K., 2016, In: RSC Advances.
Research output: Contribution to journal › Article › peer-review

Open Access
File
7 Citations (Scopus)

288 Downloads (Pure)
Periplasmic expression in and release of Fab fragments from Escherichia coli using stress minimisation
Hsu, C.-C., Thomas, O. & Overton, T., 21 Mar 2016, In: Journal of Chemical Technology and Biotechnology. 91, 3, p. 815-822
Research output: Contribution to journal › Article › peer-review

Open Access
File
13 Citations (Scopus)

715 Downloads (Pure)
Rapid enzyme regeneration results in the striking catalytic longevity of an engineered, single species, biocatalytic biofilm
Tong, X., Triscari Barberi, T., Botting, C. H., Sharma, S. V., Simmons, M., Overton, T. & Goss, R., 21 Oct 2016, In: Microbial cell factories. 15, 180.
Research output: Contribution to journal › Article › peer-review

Open Access
File
4 Citations (Scopus)

151 Downloads (Pure)
Subcritical water conversion of cellulose into glucose for bioethanol production in an integrated biorefinery process
Barros, A., Bowra, S. & Overton, T., 10 Jun 2016, European Biomass Conference and Exhibition Proceedings. 24th EUBCE ed. The European Biomass Conference and Exhibition (EUBCE), Vol. 2016. p. 1168-1172 5 p. (European Biomass Conference and Exhibition Proceedings).
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
The effects of orange juice clarification on the physiology of Escherichia coli; growth-based and flow cytometric analysis
Anvarian, A., Smith, M. & Overton, T., 16 Feb 2016, In: International Journal of Food Microbiology. 219, p. 38–43
Research output: Contribution to journal › Article › peer-review

Open Access
File
15 Citations (Scopus)

186 Downloads (Pure)
2015
Flow Cytometry of Yeasts and Other Fungi
Overton, T., 2015, Flow Cytometry in Microbiology: Technology and Applications. Wilkinson, M. (ed.). Caister Academic Press
Research output: Chapter in Book/Report/Conference proceeding › Chapter
Reduction of aerobic and lactic acid bacteria in dairy desludge using an integrated compressed CO₂ and ultrasonic process
Overton, T., Lu, T., Bains, N. & Leeke, G., Sept 2015, In: Dairy Science & Technology. 95, 5, p. 733-745
Research output: Contribution to journal › Article › peer-review

Open Access
File
2 Citations (Scopus)

180 Downloads (Pure)
Understanding and controlling the release mechanism of Escherichia coli in double W1/O/W2 emulsion globules in the presence of NaCl in the W2 phase
El Kadri, H., Overton, T., Bakalis, S. & Gkatzionis, K., 2015, In: RSC Advances. 5, 127, p. 105098-105110
Research output: Contribution to journal › Article › peer-review
10 Citations (Scopus)
2014
Flow cytometric analysis of E. coli on agar plates: implications for recombinant protein production.
Wyre, C. & Overton, T., 1 Jul 2014, In: Biotechnology Letters. 36, 7, p. 1485-1494 10 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
3 Citations (Scopus)

450 Downloads (Pure)
Recombinant protein production in bacterial hosts.
Overton, T., 1 Jun 2014, In: Drug Discovery Today. 19, 5, p. 590-601
Research output: Contribution to journal › Review article › peer-review
99 Citations (Scopus)
Use of a stress-minimisation paradigm in high cell density fed-batch Escherichia coli fermentations to optimise recombinant protein production.
Wyre, C. & Overton, T., 1 Sept 2014, In: Journal of Industrial Microbiology and Biotechnology. 41, 9, p. 1391-1404 14 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
12 Citations (Scopus)

519 Downloads (Pure)

Tim Overton, FRSB, FHEA

Research output

Search results