Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Isaac Vizcaino Caston, Catherine Kelly, Annabel Fitzgerald, Gary Leeke, Michael Jenkins, Timothy Overton

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
624 Downloads (Pure)

Abstract

We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using FT-IR spectroscopy and differential scanning calorimetry.
Original languageEnglish
Pages (from-to)101-104
JournalJournal of Bioscience and Bioengineering
Volume121
Issue number1
Early online date2 Jul 2015
DOIs
Publication statusPublished - 31 Jan 2016

Keywords

  • Polyhydroxyalkanoate
  • Dimethyl sulphoxide
  • Solvent extraction
  • Bioplastic

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