Polymer-induced biofilms for enhanced biocatalysis

Pavan Adoni, Andrey Romanyuk, Tim Overton, Francisco Fernandez-Trillo

Research output: Contribution to journalArticlepeer-review

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The intrinsic resilience of biofilms to environmental conditions makes them an attractive platform for biocatalysis, bioremediation, agriculture or consumer health. However, one of the main challenges in these areas is that beneficial bacteria are not necessarily good at biofilm formation. Currently, this problem is solved by genetic engineering or experimental evolution, techniques that can be costly and time consuming, require expertise in molecular biology and/or microbiology and, more importantly, are not suitable for all types of microorganisms or applications. Here we show that synthetic polymers can be used as an alternative, working as simple additives to nucleate the formation of biofilms. Using a combination of controlled radical polymerization and dynamic covalent chemistry, we prepare a set of synthetic polymers carrying mildly cationic, aromatic, heteroaromatic or aliphatic moieties. We then demonstrate that hydrophobic polymers induce clustering and promote biofilm formation in MC4100, a strain of Escherichia coli that forms biofilms poorly, with aromatic and heteroaromatic moieties leading to the best performing polymers. Moreover, we compare the effect of the polymers on MC4100 against PHL644, an E. coli strain that forms biofilms well due to a single point mutation which increases expression of the adhesin curli. In the presence of selected polymers, MC4100 can reach levels of biomass production and curli expression similar or higher than PHL644, demonstrating that synthetic polymers promote similar changes in microbial physiology than those introduced following genetic modification. Finally, we demonstrate that these polymers can be used to improve the performance of MC4100 biofilms in the biocatalytic transformation of 5-fluoroindole into 5-fluorotryptophan. Our results show that incubation with these synthetic polymers helps MC4100 match and even outperform PHL644 in this biotransformation, demonstrating that synthetic polymers can underpin the development of beneficial applications of biofilms.

Original languageEnglish
Pages (from-to)2592-2602
Number of pages11
JournalMaterials Horizons
Issue number10
Early online date21 Jul 2022
Publication statusPublished - 1 Oct 2022

Bibliographical note

Funding Information:
P. F.-T. thanks the University of Birmingham for the John Evans Fellowship and the Spanish Ministerio de Educación, Cultura y Deporte for a Beatriz Galindo Award [BEAGAL18/00142]. P. A. thanks the BBSRC's Midlands Integrative Biosciences Training Partnership MIBTP (BB/M01116X/1) for a PhD scholarship and A. R. thanks the European Union's Horizon 2020 Research and Innovation Programme (Marie Sklodowska-Curie Grant Agreement No. 795082).

Publisher Copyright:
© 2022 The Royal Society of Chemistry.


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  • Polymer-induced biofilms for enhanced biocatalysis

    Adoni, P., Romanyuk, A., Overton, T. & Fernandez-Trillo, F., 24 Mar 2022, ChemRxiv, (ChemRxiv).

    Research output: Working paper/PreprintWorking paper

  • Methods and Products

    Fernandez-Trillo, F., Overton, T., Adoni, P. & Huneidi, O., 21 Oct 2021, Patent No. WO2021209765A2, 16 Apr 2021, Priority date 17 Apr 2021, Priority No. GB2005605.7

    Research output: Patent


    Fernandez-Trillo, F.

    European Commission


    Project: EU

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