Iridium nanoparticles for multichannel luminescence lifetime imaging, mapping localization in live cancer cells

Research output: Contribution to journalArticle

Standard

Iridium nanoparticles for multichannel luminescence lifetime imaging, mapping localization in live cancer cells. / King, Siobhan M; Claire, Sunil; Teixeira, Rodolfo I; Dosumu, Abiola N; Carrod, Andrew J; Dehghani, Hamid; Hannon, Michael J; Ward, Andrew D; Bicknell, Roy; Botchway, Stanley W; Hodges, Nikolas J; Pikramenou, Zoe.

In: Journal of the American Chemical Society, Vol. 140, No. 32, 15.08.2018, p. 10242-10249.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

King, Siobhan M ; Claire, Sunil ; Teixeira, Rodolfo I ; Dosumu, Abiola N ; Carrod, Andrew J ; Dehghani, Hamid ; Hannon, Michael J ; Ward, Andrew D ; Bicknell, Roy ; Botchway, Stanley W ; Hodges, Nikolas J ; Pikramenou, Zoe. / Iridium nanoparticles for multichannel luminescence lifetime imaging, mapping localization in live cancer cells. In: Journal of the American Chemical Society. 2018 ; Vol. 140, No. 32. pp. 10242-10249.

Bibtex

@article{df97fe066afe4513ab90d6d02e32c148,
title = "Iridium nanoparticles for multichannel luminescence lifetime imaging, mapping localization in live cancer cells",
abstract = "The development of long-lived luminescent nanoparticles for lifetime imaging is of wide interest as luminescence lifetime is environmentally sensitive detection independent of probe concentration. We report novel iridium-coated gold nanoparticles as probes for multiphoton lifetime imaging with characteristic long luminescent lifetimes based on iridium luminescence in the range of hundreds of nanoseconds and a short signal on the scale of picoseconds based on gold allowing multichannel detection. The tailor-made IrC6 complex forms stable, water-soluble gold nanoparticles (AuNPs) of 13, 25, and 100 nm, bearing 1400, 3200, and 22 000 IrC6 complexes per AuNP, respectively. The sensitivity of the iridium signal on the environment of the cell is evidenced with an observed variation of lifetimes. Clusters of iridium nanoparticles show lifetimes from 450 to 590 ns while lifetimes of 660 and 740 ns are an average of different points in the cytoplasm and nucleus. Independent luminescence lifetime studies of the nanoparticles in different media and under aggregation conditions postulate that the unusual long lifetimes observed can be attributed to interaction with proteins rather than nanoparticle aggregation. Total internal reflection fluorescence microscopy (TIRF), confocal microscopy studies and 3D luminescence lifetime stacks confirm the presence of bright, nonaggregated nanoparticles inside the cell. Inductively coupled plasma mass spectrometry (ICPMS) analysis further supports the presence of the nanoparticles in cells. The iridium-coated nanoparticles provide new nanoprobes for lifetime detection with dual channel monitoring. The combination of the sensitivity of the iridium signal to the cell environment together with the nanoscaffold to guide delivery offer opportunities for iridium nanoparticles for targeting and tracking in in vivo models.",
author = "King, {Siobhan M} and Sunil Claire and Teixeira, {Rodolfo I} and Dosumu, {Abiola N} and Carrod, {Andrew J} and Hamid Dehghani and Hannon, {Michael J} and Ward, {Andrew D} and Roy Bicknell and Botchway, {Stanley W} and Hodges, {Nikolas J} and Zoe Pikramenou",
year = "2018",
month = aug,
day = "15",
doi = "10.1021/jacs.8b05105",
language = "English",
volume = "140",
pages = "10242--10249",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "32",

}

RIS

TY - JOUR

T1 - Iridium nanoparticles for multichannel luminescence lifetime imaging, mapping localization in live cancer cells

AU - King, Siobhan M

AU - Claire, Sunil

AU - Teixeira, Rodolfo I

AU - Dosumu, Abiola N

AU - Carrod, Andrew J

AU - Dehghani, Hamid

AU - Hannon, Michael J

AU - Ward, Andrew D

AU - Bicknell, Roy

AU - Botchway, Stanley W

AU - Hodges, Nikolas J

AU - Pikramenou, Zoe

PY - 2018/8/15

Y1 - 2018/8/15

N2 - The development of long-lived luminescent nanoparticles for lifetime imaging is of wide interest as luminescence lifetime is environmentally sensitive detection independent of probe concentration. We report novel iridium-coated gold nanoparticles as probes for multiphoton lifetime imaging with characteristic long luminescent lifetimes based on iridium luminescence in the range of hundreds of nanoseconds and a short signal on the scale of picoseconds based on gold allowing multichannel detection. The tailor-made IrC6 complex forms stable, water-soluble gold nanoparticles (AuNPs) of 13, 25, and 100 nm, bearing 1400, 3200, and 22 000 IrC6 complexes per AuNP, respectively. The sensitivity of the iridium signal on the environment of the cell is evidenced with an observed variation of lifetimes. Clusters of iridium nanoparticles show lifetimes from 450 to 590 ns while lifetimes of 660 and 740 ns are an average of different points in the cytoplasm and nucleus. Independent luminescence lifetime studies of the nanoparticles in different media and under aggregation conditions postulate that the unusual long lifetimes observed can be attributed to interaction with proteins rather than nanoparticle aggregation. Total internal reflection fluorescence microscopy (TIRF), confocal microscopy studies and 3D luminescence lifetime stacks confirm the presence of bright, nonaggregated nanoparticles inside the cell. Inductively coupled plasma mass spectrometry (ICPMS) analysis further supports the presence of the nanoparticles in cells. The iridium-coated nanoparticles provide new nanoprobes for lifetime detection with dual channel monitoring. The combination of the sensitivity of the iridium signal to the cell environment together with the nanoscaffold to guide delivery offer opportunities for iridium nanoparticles for targeting and tracking in in vivo models.

AB - The development of long-lived luminescent nanoparticles for lifetime imaging is of wide interest as luminescence lifetime is environmentally sensitive detection independent of probe concentration. We report novel iridium-coated gold nanoparticles as probes for multiphoton lifetime imaging with characteristic long luminescent lifetimes based on iridium luminescence in the range of hundreds of nanoseconds and a short signal on the scale of picoseconds based on gold allowing multichannel detection. The tailor-made IrC6 complex forms stable, water-soluble gold nanoparticles (AuNPs) of 13, 25, and 100 nm, bearing 1400, 3200, and 22 000 IrC6 complexes per AuNP, respectively. The sensitivity of the iridium signal on the environment of the cell is evidenced with an observed variation of lifetimes. Clusters of iridium nanoparticles show lifetimes from 450 to 590 ns while lifetimes of 660 and 740 ns are an average of different points in the cytoplasm and nucleus. Independent luminescence lifetime studies of the nanoparticles in different media and under aggregation conditions postulate that the unusual long lifetimes observed can be attributed to interaction with proteins rather than nanoparticle aggregation. Total internal reflection fluorescence microscopy (TIRF), confocal microscopy studies and 3D luminescence lifetime stacks confirm the presence of bright, nonaggregated nanoparticles inside the cell. Inductively coupled plasma mass spectrometry (ICPMS) analysis further supports the presence of the nanoparticles in cells. The iridium-coated nanoparticles provide new nanoprobes for lifetime detection with dual channel monitoring. The combination of the sensitivity of the iridium signal to the cell environment together with the nanoscaffold to guide delivery offer opportunities for iridium nanoparticles for targeting and tracking in in vivo models.

U2 - 10.1021/jacs.8b05105

DO - 10.1021/jacs.8b05105

M3 - Article

C2 - 30032598

VL - 140

SP - 10242

EP - 10249

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 32

ER -