Imaging workflow and calibration for CT-guided time-domain fluorescence tomography
Research output: Contribution to journal › Article
Authors
Colleges, School and Institutes
Abstract
In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
Details
Original language | English |
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Pages (from-to) | 3021-3036 |
Number of pages | 16 |
Journal | Biomedical Optics Express |
Volume | 2 |
Issue number | 11 |
Publication status | Published - 1 Jan 2011 |