Imaging workflow and calibration for CT-guided time-domain fluorescence tomography

Research output: Contribution to journalArticle


  • KM Tichauer
  • RW Holt
  • F El-Ghussein
  • F Leblond
  • BW Pogue

Colleges, School and Institutes


In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.


Original languageEnglish
Pages (from-to)3021-3036
Number of pages16
JournalBiomedical Optics Express
Issue number11
Publication statusPublished - 1 Jan 2011