Imaging workflow and calibration for CT-guided time-domain fluorescence tomography

KM Tichauer, RW Holt, F El-Ghussein, Qun Zhu, Hamid Dehghani, F Leblond, BW Pogue

Research output: Contribution to journalArticle

27 Citations (Scopus)


In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
Original languageEnglish
Pages (from-to)3021-3036
Number of pages16
JournalBiomedical Optics Express
Issue number11
Publication statusPublished - 1 Jan 2011


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