Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children: the Generation R Study

Diana van den Heuvel, Michelle A E Jansen, Andrew I Bell, Alan B Rickinson, Vincent W V Jaddoe, Jacques J M van Dongen, Henriette A Moll, Menno C van Zelm

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV(+) adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27(+)IgG(+), CD27(+)IgA(+), and CD27(-)IgA(+) memory B cells than in IgM-only, natural effector, and CD27(-)IgG(+) B cells. The blood counts of IgM-only, CD27(+)IgA(+), CD27(-)IgG(+), and CD27(+)IgG(+) memory B cells were significantly lower in EBV(+) children than in uninfected controls at 14 mo of age-the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27(-)IgA(+)), and EBV infection results in a transient depletion of these cells in young children.

Original languageEnglish
Pages (from-to)949-956
JournalJournal of Leukocyte Biology
Volume101
Issue number4
Early online date7 Nov 2016
DOIs
Publication statusPublished - Apr 2017

Keywords

  • pediatric infection
  • herpes virus
  • humoral immunity
  • Epstein-Barr virus

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