Projects per year
The temporal transcriptomic responses in liver of Platichthys flesus to model environmental pollutants were studied over a 16-day time span after intraperitoneal injection with cadmium chloride (50 mu g/kg in saline), 3-methylcholanthrene (25 mg/kg in olive oil), Aroclor 1254 (50 mg/kg in olive oil), tert-butylhydroperoxide (5 mg/kg in saline), Lindane (25 mg/kg in olive oil), perfluoro-octanoic acid (100 mg/kg in olive oil) and their vehicles, olive oil (1 ml/kg) or saline (0.9%). Statistical, gene ontology and supervised analysis clearly demonstrated the progression from acute effects, biological responses to and recovery from the treatments. Key biological processes disturbed by the individual treatments were characterised by gene ontology analyses and individual toxicant-responsive genes and pathways were identified by supervised analyses. Responses to the polyaromatic and chlorinated aromatic compounds showed a degree of commonality but were distinguishable and they were clearly segregated from the responses to the pro-oxidants cadmium and the organic hydroperoxide, as well as from the peroxisomal proliferator, perfluoro-octanoic acid. This study demonstrated the utility of the microarray technique in the identification of toxicant-responsive genes and in discrimination between modes of toxicant action. (c) 2008 Elsevier B.V. All rights reserved.
|Number of pages||9|
|Early online date||15 Aug 2008|
|Publication status||Published - 11 Nov 2008|
- Peroxisome proliferators
- Oxidative stress
- Polycyclic aromatic hydrocarbon
FingerprintDive into the research topics of 'Transcriptomic responses of European flounder (Platichthys flesus) to model toxicants'. Together they form a unique fingerprint.
- 1 Finished
Identifying and Defining the Bases of Individual and Population Susceptibility and Adaption to Environmental Pollutants in Fish: An Integrated Transcriptomic and Metabolomic Approach
Chipman, K., Falciani, F., Viant, M. & Minchin, S.
Natural Environment Research Council
1/04/05 → 30/11/08
Project: Research Councils