The expression of active CD11b monocytes in blood and disease progression in amyotrophic lateral sclerosis

Ozlem Yildiz, Jonathan Schroth, Vittoria Lombardi, Valentina Pucino, Yoana Bobeva, Ping Kei Yip, Klaus Schmierer, Claudio Mauro, Timothy Tree, Sian Mari Henson, Andrea Malaspina

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Abstract

Monocytes expressing the inflammation suppressing active CD11b, a beta2 integrin, may regulate neuroinflammation and modify clinical outcomes in amyotrophic lateral sclerosis (ALS). In this single site, retrospective study, peripheral blood mononuclear cells from 38 individuals living with ALS and 20 non-neurological controls (NNC) were investigated using flow cytometry to study active CD11b integrin classical (CM), intermediate (IM) and non-classical (NCM) monocytes during ALS progression. Seventeen ALS participants were sampled at the baseline (V1) and at two additional time points (V2 and V3) for longitudinal analysis. Active CD11b+ CM frequencies increased steeply between the baseline and V3 (ANOVA repeated measurement, p < 0.001), and the V2/V1 ratio negatively correlated with the disease progression rate, similar to higher frequencies of active CD11b+ NCM at the baseline (R = −0.6567; p = 0.0031 and R = 0.3862; p = 0.0168, respectively). CD11b NCM, clinical covariates and neurofilament light-chain plasma concentration at the baseline predicted shorter survival in a multivariable and univariate analysis (CD11b NCM—HR: 1.05, CI: 1.01–1.11, p = 0.013. Log rank: above median: 43 months and below median: 21.22 months; p = 0.0022). Blood samples with the highest frequencies of active CD11b+ IM and NCM contained the lowest concentrations of soluble CD11b. Our preliminary data suggest that the levels of active CD11b+ monocytes and NCM in the blood predict different clinical outcomes in ALS.
Original languageEnglish
Article number3370
Number of pages13
JournalInternational Journal of Molecular Sciences
Volume23
Issue number6
DOIs
Publication statusPublished - 21 Mar 2022

Keywords

  • amyotrophic lateral sclerosis
  • monocytes
  • CD11b
  • beta2 integrin

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