Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates

E. M. Smith; L. E. Green; G. F. Medley; H. E. Bird; L. K. Fox; Y. H. Schukken; J. V. Kruze; A. J. Bradley; R. N. Zadoks; C. G. Dowson

doi:10.1128/JCM.43.9.4737-4743.2005

Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates

E. M. Smith, L. E. Green^*, G. F. Medley, H. E. Bird, L. K. Fox, Y. H. Schukken, J. V. Kruze, A. J. Bradley, R. N. Zadoks, C. G. Dowson

^*Corresponding author for this work

Life and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

133 Citations (Scopus)

Abstract

A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the differentiation of bovine S. aureus isolates from various sites (milk, teat skin, milking machine unit liners, hands, and bedding) and countries. The theory of the host specificity of S. aureus is supported by the detection of a previously undescribed clonal complex that comprised 87.4% of the isolates studied, with representatives from all geographic locations investigated. This suggests that a single clonal group has achieved a widespread distribution and is responsible for the majority of infections. Some sequence types (STs; ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05) associated with milk or teat skin.

Original language	English
Pages (from-to)	4737-4743
Number of pages	7
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	9
DOIs	https://doi.org/10.1128/JCM.43.9.4737-4743.2005
Publication status	Published - Sept 2005

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/JCM.43.9.4737-4743.2005

Cite this

@article{2ef05cf3be844c68a37467df92876660,

title = "Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates",

abstract = "A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the differentiation of bovine S. aureus isolates from various sites (milk, teat skin, milking machine unit liners, hands, and bedding) and countries. The theory of the host specificity of S. aureus is supported by the detection of a previously undescribed clonal complex that comprised 87.4% of the isolates studied, with representatives from all geographic locations investigated. This suggests that a single clonal group has achieved a widespread distribution and is responsible for the majority of infections. Some sequence types (STs; ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05) associated with milk or teat skin.",

author = "Smith, {E. M.} and Green, {L. E.} and Medley, {G. F.} and Bird, {H. E.} and Fox, {L. K.} and Schukken, {Y. H.} and Kruze, {J. V.} and Bradley, {A. J.} and Zadoks, {R. N.} and Dowson, {C. G.}",

year = "2005",

month = sep,

doi = "10.1128/JCM.43.9.4737-4743.2005",

language = "English",

volume = "43",

pages = "4737--4743",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "9",

}

TY - JOUR

T1 - Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates

AU - Smith, E. M.

AU - Green, L. E.

AU - Medley, G. F.

AU - Bird, H. E.

AU - Fox, L. K.

AU - Schukken, Y. H.

AU - Kruze, J. V.

AU - Bradley, A. J.

AU - Zadoks, R. N.

AU - Dowson, C. G.

PY - 2005/9

Y1 - 2005/9

N2 - A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the differentiation of bovine S. aureus isolates from various sites (milk, teat skin, milking machine unit liners, hands, and bedding) and countries. The theory of the host specificity of S. aureus is supported by the detection of a previously undescribed clonal complex that comprised 87.4% of the isolates studied, with representatives from all geographic locations investigated. This suggests that a single clonal group has achieved a widespread distribution and is responsible for the majority of infections. Some sequence types (STs; ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05) associated with milk or teat skin.

AB - A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the differentiation of bovine S. aureus isolates from various sites (milk, teat skin, milking machine unit liners, hands, and bedding) and countries. The theory of the host specificity of S. aureus is supported by the detection of a previously undescribed clonal complex that comprised 87.4% of the isolates studied, with representatives from all geographic locations investigated. This suggests that a single clonal group has achieved a widespread distribution and is responsible for the majority of infections. Some sequence types (STs; ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05) associated with milk or teat skin.

UR - http://www.scopus.com/inward/record.url?scp=24744467373&partnerID=8YFLogxK

U2 - 10.1128/JCM.43.9.4737-4743.2005

DO - 10.1128/JCM.43.9.4737-4743.2005

M3 - Article

C2 - 16145135

AN - SCOPUS:24744467373

SN - 0095-1137

VL - 43

SP - 4737

EP - 4743

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates

Abstract

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this