Flow Linear Dichroism of Some Prototypical Proteins

BM Bulheller, A Rodger, MR Hicks, Timothy Dafforn, LC Serpell, KE Marshall, EHC Bromley, PJS King, KJ Channon, DN Woolfson, JD Hirst

Research output: Contribution to journalArticle

32 Citations (Scopus)


Flow linear dichroism (LD) spectroscopy provides information on the orientation of molecules in solution and hence on the relative orientation of parts of molecules. Long molecules such as fibrous proteins can be aligned in Couette flow cells and characterized using LD. We have measured using Couette flow and calculated from first principles the LD of proteins representing prototypical secondary structure classes: a self-assembling fiber,and tropomyosin (all-alpha-helical), FtsZ (an alpha beta protein), an amyloid fibril (beta-sheet), and Collagen [poly(proline)II helices]. the combination of calculation and experiment allows elucidation of the protein orientation in the Couette flow and the orientation of chromophores within the protein fibers.
Original languageEnglish
Pages (from-to)13305-13314
Number of pages10
JournalJournal of the American Chemical Society
Issue number37
Publication statusPublished - 1 Sept 2009


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