A DNA expression array to detect toxic response in European flounder (Platichthys flesus)

Timothy Williams, Karl Gensberg, Stephen Minchin, James Chipman

Research output: Contribution to journalArticlepeer-review

198 Citations (Scopus)
10 Downloads (Pure)


As a first stage in developing a DNA array-based approach to investigating the effects of pollutants on an environmentally relevant European fish species, we have constructed a 160-gene custom microarray for European flounder. Degenerate primers were used to amplify 110 different fragments of stress-related and other genes from European flounder cDNA and genomic DNA. Additionally, 22 fragments were obtained by suppressive subtractive hybridisation (SSH). These fragments were cloned and sequenced, then, with additional control genes, used to create a cDNA microarray for flounder. After optimisation of the arraying process, hepatic mRNA was isolated from flounder caught in the polluted Tyne and relatively unpolluted Alde estuaries. Fluorescent cDNA probes were synthesised from the mRNA and used in dual-colour hybridisations to the microarray. A number of transcripts were differentially expressed between Tyne and Alde female flounder but these changes were not significant, due to high inter-individual variation. However, in comparisons between Tyne and Alde male flounder, 11 transcripts were found to significantly differ in expression (P
Original languageEnglish
Pages (from-to)141-157
Number of pages17
JournalAquatic Toxicology
Issue number2
Publication statusPublished - 29 Oct 2003


  • CYP1A
  • toxicogenomics
  • real-time PCR
  • microarray
  • flounder
  • eDNA array


Dive into the research topics of 'A DNA expression array to detect toxic response in European flounder (Platichthys flesus)'. Together they form a unique fingerprint.

Cite this