Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking
Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review
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Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking. / Wiggins, Benjamin G; Stamataki, Zania; Lalor, Patricia F.
T-Cell Trafficking: Methods and Protocols. ed. / George Edward Rainger; Helen M. Mcgettrick. Vol. 1591 Springer, 2017. p. 177-194 (Methods in Molecular Biology).Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review
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TY - CHAP
T1 - Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking
AU - Wiggins, Benjamin G
AU - Stamataki, Zania
AU - Lalor, Patricia F
PY - 2017/3/28
Y1 - 2017/3/28
N2 - Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.
AB - Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.
KW - Journal Article
KW - antibody
KW - human
KW - inflammation
KW - liver
KW - lymphocyte
KW - organ culture
U2 - 10.1007/978-1-4939-6931-9_13
DO - 10.1007/978-1-4939-6931-9_13
M3 - Chapter (peer-reviewed)
C2 - 28349483
SN - 978-1-4939-6929-6
VL - 1591
T3 - Methods in Molecular Biology
SP - 177
EP - 194
BT - T-Cell Trafficking:
A2 - Rainger, George Edward
A2 - Mcgettrick, Helen M.
PB - Springer
ER -