Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Benjamin G Wiggins; Zania Stamataki; Patricia F Lalor

doi:10.1007/978-1-4939-6931-9_13

Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Benjamin G Wiggins, Zania Stamataki, Patricia F Lalor

Immunology and Immunotherapy

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.

Original language	English
Title of host publication	T-Cell Trafficking:
Subtitle of host publication	Methods and Protocols
Editors	George Edward Rainger, Helen M. Mcgettrick
Publisher	Springer
Pages	177-194
Number of pages	18
Volume	1591
ISBN (Electronic)	978-1-4939-6931-9
ISBN (Print)	978-1-4939-6929-6
DOIs	https://doi.org/10.1007/978-1-4939-6931-9_13
Publication status	Published - 28 Mar 2017

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
ISSN (Print)	1064-3745

Keywords

Journal Article
antibody
human
inflammation
liver
lymphocyte
organ culture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-6931-9_13Licence: None: All rights reserved

https://link.springer.com/protocol/10.1007%2F978-1-4939-6931-9_13Licence: None: All rights reserved

Cite this

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abstract = "Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.",

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Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking. / Wiggins, Benjamin G; Stamataki, Zania ; Lalor, Patricia F.
T-Cell Trafficking: Methods and Protocols. ed. / George Edward Rainger; Helen M. Mcgettrick. Vol. 1591 Springer, 2017. p. 177-194 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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KW - inflammation

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KW - lymphocyte

KW - organ culture

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Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Abstract

Publication series

Keywords

UN SDGs

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