Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Research output: Contribution to journalArticlepeer-review

Authors

  • Tom Robinson
  • Prashant Valluri
  • Hugh B. Manning
  • Ian Munro
  • Clifford B. Talbot
  • Christopher Dunsby
  • John F. Eccleston
  • Geoff S. Baldwin
  • Mark A. A. Neil
  • Andrew J. de Mello
  • Paul M. W. French

Colleges, School and Institutes

Abstract

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 +/- 0.4 ms were achieved with the single-layer microfluidic device.

Details

Original languageEnglish
Pages (from-to)1887-1889
Number of pages3
JournalOptics Letters
Volume33
Issue number16
Publication statusPublished - 15 Aug 2008