Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 +/- 0.4 ms were achieved with the single-layer microfluidic device.
|Number of pages||3|
|Publication status||Published - 15 Aug 2008|