Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Tom Robinson; Prashant Valluri; Hugh B. Manning; Dylan Owen; Ian Munro; Clifford B. Talbot; Christopher Dunsby; John F. Eccleston; Geoff S. Baldwin; Mark A. A. Neil; Andrew J. de Mello; Paul M. W. French

doi:10.1364/OL.33.001887

Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Tom Robinson, Prashant Valluri, Hugh B. Manning, Dylan Owen, Ian Munro, Clifford B. Talbot, Christopher Dunsby, John F. Eccleston, Geoff S. Baldwin, Mark A. A. Neil, Andrew J. de Mello, Paul M. W. French

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 +/- 0.4 ms were achieved with the single-layer microfluidic device.

Original language	English
Pages (from-to)	1887-1889
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	16
DOIs	https://doi.org/10.1364/OL.33.001887
Publication status	Published - 15 Aug 2008

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title = "Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging",

abstract = "Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 +/- 0.4 ms were achieved with the single-layer microfluidic device.",

author = "Tom Robinson and Prashant Valluri and Manning, {Hugh B.} and Dylan Owen and Ian Munro and Talbot, {Clifford B.} and Christopher Dunsby and Eccleston, {John F.} and Baldwin, {Geoff S.} and Neil, {Mark A. A.} and {de Mello}, {Andrew J.} and French, {Paul M. W.}",

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AU - Robinson, Tom

AU - Valluri, Prashant

AU - Manning, Hugh B.

AU - Owen, Dylan

AU - Munro, Ian

AU - Talbot, Clifford B.

AU - Dunsby, Christopher

AU - Eccleston, John F.

AU - Baldwin, Geoff S.

AU - Neil, Mark A. A.

AU - de Mello, Andrew J.

AU - French, Paul M. W.

PY - 2008/8/15

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N2 - Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 +/- 0.4 ms were achieved with the single-layer microfluidic device.

AB - Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 +/- 0.4 ms were achieved with the single-layer microfluidic device.

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DO - 10.1364/OL.33.001887

M3 - Article

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VL - 33

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EP - 1889

JO - Optics Letters

JF - Optics Letters

IS - 16

ER -

Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

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