Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

Research output: Contribution to journalArticle

Authors

  • Jake G. Carter
  • Lorea Orueta Iturbe
  • Ian R. Carter
  • Craig D. Southern
  • Marium Rana
  • Andrew Bosworth

Abstract

We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.

Details

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalmedRxiv
Volume2021
Publication statusPublished - 4 Jan 2021