Sub-5-minute detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

Jake G. Carter, Lorea Orueta Iturbe, Jean-Louis H. A. Duprey, Ian R. Carter, Craig D. Southern, Marium Rana, Andrew Bosworth, Andrew D. Beggs, Matthew R. Hicks, James H. R. Tucker, Timothy R. Dafforn

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Abstract

We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalmedRxiv
Volume2021
DOIs
Publication statusPublished - 4 Jan 2021

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