Studies of the Escherichia coli Rsd-sigma70 complex.

Research output: Contribution to journalArticle

Authors

  • Lars Westblade
  • LL Ilag
  • A Kolb
  • CV Robinson

Colleges, School and Institutes

Abstract

Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.

Details

Original languageEnglish
Pages (from-to)685-92
Number of pages8
JournalJournal of Molecular Biology
Volume335
Issue number3
Publication statusPublished - 16 Jan 2004

Keywords

  • transcription initiation, Escherichia coli, sigma-factors, stoichiometry, anti-sigma factors