Abstract
Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.
Original language | English |
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Pages (from-to) | 685-92 |
Number of pages | 8 |
Journal | Journal of Molecular Biology |
Volume | 335 |
Issue number | 3 |
DOIs | |
Publication status | Published - 16 Jan 2004 |
Keywords
- transcription initiation
- Escherichia coli
- sigma-factors
- stoichiometry
- anti-sigma factors