Studies of the Escherichia coli Rsd-sigma70 complex.
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Colleges, School and Institutes
Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.
|Number of pages||8|
|Journal||Journal of Molecular Biology|
|Publication status||Published - 16 Jan 2004|
- transcription initiation, Escherichia coli, sigma-factors, stoichiometry, anti-sigma factors