Studies of the Escherichia coli Rsd-sigma70 complex.

Lars Westblade; LL Ilag; Andrew Powell; A Kolb; CV Robinson; Stephen Busby

doi:10.1016/j.jmb.2003.11.004

Studies of the Escherichia coli Rsd-sigma70 complex.

Lars Westblade, LL Ilag, Andrew Powell, A Kolb, CV Robinson, Stephen Busby

Biosciences

Research output: Contribution to journal › Article

35 Citations (Scopus)

Abstract

Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.

Original language	English
Pages (from-to)	685-92
Number of pages	8
Journal	Journal of Molecular Biology
Volume	335
Issue number	3
DOIs	https://doi.org/10.1016/j.jmb.2003.11.004
Publication status	Published - 16 Jan 2004

Keywords

transcription initiation
Escherichia coli
sigma-factors
stoichiometry
anti-sigma factors

Access to Document

10.1016/j.jmb.2003.11.004

Cite this

@article{a5267751411147c281520dd7d4d87059,

title = "Studies of the Escherichia coli Rsd-sigma70 complex.",

abstract = "Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.",

keywords = "transcription initiation, Escherichia coli, sigma-factors, stoichiometry, anti-sigma factors",

author = "Lars Westblade and LL Ilag and Andrew Powell and A Kolb and CV Robinson and Stephen Busby",

year = "2004",

month = jan,

day = "16",

doi = "10.1016/j.jmb.2003.11.004",

language = "English",

volume = "335",

pages = "685--92",

journal = "Journal of Molecular Biology",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Studies of the Escherichia coli Rsd-sigma70 complex.

AU - Westblade, Lars

AU - Ilag, LL

AU - Powell, Andrew

AU - Kolb, A

AU - Robinson, CV

AU - Busby, Stephen

PY - 2004/1/16

Y1 - 2004/1/16

N2 - Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.

AB - Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.

KW - transcription initiation

KW - Escherichia coli

KW - sigma-factors

KW - stoichiometry

KW - anti-sigma factors

U2 - 10.1016/j.jmb.2003.11.004

DO - 10.1016/j.jmb.2003.11.004

M3 - Article

C2 - 14687566

VL - 335

SP - 685

EP - 692

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 3

ER -

Studies of the Escherichia coli Rsd-sigma70 complex.

Abstract

Keywords

Access to Document

Fingerprint

Cite this