Selective glycoprotein detection through covalent templating and allosteric click-imprinting

Research output: Contribution to journalArticlepeer-review

Colleges, School and Institutes

Abstract

Many glycoproteins are intimately linked to the onset and progression of numerous heritable or acquired diseases of humans, including cancer. Indeed the recognition of specific glycoproteins remains a significant challenge in analytical method and diagnostic development. Herein, a hierarchical bottom-up route exploiting reversible covalent interactions with boronic acids and so-called click chemistry for the fabrication of glycoprotein selective surfaces that surmount current antibody constraints is described. The self-assembled and imprinted surfaces, containing specific glycoprotein molecular recognition nanocavities, confer high binding affinities, nanomolar sensitivity, exceptional glycoprotein specificity and selectivity with as high as 30 fold selectivity for prostate specific antigen (PSA) over other glycoproteins. This synthetic, robust and highly selective recognition platform can be used in complex biological media and be recycled multiple times with no performance decrement.

Details

Original languageEnglish
Pages (from-to)5114-5119
Number of pages6
JournalChemical Science
Volume6
Issue number9
Early online date17 Jun 2015
Publication statusPublished - 1 Sep 2015

ASJC Scopus subject areas