Production and release of toxins A and B by Clostridium difficile

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Authors

Colleges, School and Institutes

Abstract

The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

Details

Original languageEnglish
Pages (from-to)385-91
Number of pages7
JournalJournal of Medical Microbiology
Volume18
Issue number3
Publication statusPublished - Dec 1984

Keywords

  • Bacterial Proteins, Bacterial Toxins, Clostridium, Cytotoxins, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Immunoelectrophoresis, Two-Dimensional