Production and release of toxins A and B by Clostridium difficile

J M Ketley; S C Haslam; T J Mitchell; J Stephen; D C Candy; D W Burdon

doi:10.1099/00222615-18-3-385

Production and release of toxins A and B by Clostridium difficile

J M Ketley, S C Haslam, T J Mitchell, J Stephen, D C Candy, D W Burdon

Microbiology and Infection

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14 Citations (Scopus)

Abstract

The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

Original language	English
Pages (from-to)	385-91
Number of pages	7
Journal	Journal of Medical Microbiology
Volume	18
Issue number	3
DOIs	https://doi.org/10.1099/00222615-18-3-385
Publication status	Published - Dec 1984

Keywords

Bacterial Proteins
Bacterial Toxins
Clostridium
Cytotoxins
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Immunoelectrophoresis, Two-Dimensional

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10.1099/00222615-18-3-385

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title = "Production and release of toxins A and B by Clostridium difficile",

abstract = "The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.",

keywords = "Bacterial Proteins, Bacterial Toxins, Clostridium, Cytotoxins, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Immunoelectrophoresis, Two-Dimensional",

author = "Ketley, {J M} and Haslam, {S C} and Mitchell, {T J} and J Stephen and Candy, {D C} and Burdon, {D W}",

year = "1984",

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doi = "10.1099/00222615-18-3-385",

language = "English",

volume = "18",

pages = "385--91",

journal = "Journal of Medical Microbiology",

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T1 - Production and release of toxins A and B by Clostridium difficile

AU - Ketley, J M

AU - Haslam, S C

AU - Mitchell, T J

AU - Stephen, J

AU - Candy, D C

AU - Burdon, D W

PY - 1984/12

Y1 - 1984/12

N2 - The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

AB - The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

KW - Bacterial Proteins

KW - Bacterial Toxins

KW - Clostridium

KW - Cytotoxins

KW - Enzyme-Linked Immunosorbent Assay

KW - Fluorescent Antibody Technique

KW - Immunoelectrophoresis, Two-Dimensional

U2 - 10.1099/00222615-18-3-385

DO - 10.1099/00222615-18-3-385

M3 - Article

C2 - 6389875

SN - 0022-2615

VL - 18

SP - 385

EP - 391

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

IS - 3

ER -

Production and release of toxins A and B by Clostridium difficile

Abstract

Keywords

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