Involvement of the intermediate filament protein cytokeratin-18 in actin pedestal formation during EPEC infection

Research output: Contribution to journalArticle

Authors

  • M Batchelor
  • J Guignot
  • A Patel
  • N Cumings
  • Jennifer Cleary
  • DW Holden
  • I Connerton
  • G Frankel

Colleges, School and Institutes

Abstract

While remaining extracellular, enteropathogenic Escherichia coli (EPEC) establish direct links with the cytoskeleton of the target epithelial cell leading to the formation of actin-rich pedestals underneath attached bacteria. The translocated adaptor protein Tir forms the transmembrane bridge between the cytoskeleton and the bacterium; the extracellular domain of Tir functions as a receptor for the bacterial adhesin intimin, while the intracellular amino and carboxy termini interact with a number of focal adhesion and other cytoskeletal proteins; and recruitment of some is dependent on phosphorylation of Tyr 474. Using Tir as bait and HeLa cell cDNA library as prey in a yeast two-hybrid screen, we identified cytokeratin 18 as a novel Tir partner protein. Cytokeratin 18 is recruited to the EPEC-induced pedestal and has a direct role in actin accretion and cytoskeleton reorganization. This study is the first to implicate intermediate filaments in microfilament reorganization following EPEC infection.

Details

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalEMBO Reports
Volume5
Issue number1
Publication statusPublished - 1 Jan 2004