In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Authors

  • Benjamin G Wiggins
  • Konstantinos Aliazis
  • Gary Reynolds

Colleges, School and Institutes

External organisations

  • Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK.
  • Centre for Liver Research, Institute for Immunology and Immunotherapy, University of Birmingham, Birmingham, B15 2TT, UK.
  • Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK. z.stamataki@bham.ac.uk.

Abstract

The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.

Details

Original languageEnglish
Title of host publicationT-Cell Trafficking:
Subtitle of host publicationMethods and Protocols
EditorsGeorge Edward Rainger, Helen M. Mcgettrick
Publication statusPublished - 28 Mar 2017

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745

Keywords

  • Journal Article, adhesion, cell culture , coculture , cytokines , inflammation , liver , liver wedge , lymphocytes , migration , perfusion , peristaltic pump , T cells