In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma
Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review
Colleges, School and Institutes
- Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK.
- Centre for Liver Research, Institute for Immunology and Immunotherapy, University of Birmingham, Birmingham, B15 2TT, UK.
- Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, B15 2TT, UK. email@example.com.
The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.
|Title of host publication||T-Cell Trafficking:|
|Subtitle of host publication||Methods and Protocols|
|Editors||George Edward Rainger, Helen M. Mcgettrick|
|Publication status||Published - 28 Mar 2017|
|Name||Methods in Molecular Biology|
- Journal Article, adhesion, cell culture , coculture , cytokines , inflammation , liver , liver wedge , lymphocytes , migration , perfusion , peristaltic pump , T cells