In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

Benjamin G Wiggins, Konstantinos Aliazis, Scott P Davies, Gideon Hirschfield, Patricia F Lalor, Gary Reynolds, Zania Stamataki

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.

Original languageEnglish
Title of host publicationT-Cell Trafficking:
Subtitle of host publicationMethods and Protocols
EditorsGeorge Edward Rainger, Helen M. Mcgettrick
Number of pages20
ISBN (Electronic)9781493969319
ISBN (Print)9781493969296
Publication statusPublished - 28 Mar 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Journal Article
  • adhesion
  • cell culture
  • coculture
  • cytokines
  • inflammation
  • liver
  • liver wedge
  • lymphocytes
  • migration
  • perfusion
  • peristaltic pump
  • T cells


Dive into the research topics of 'In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma'. Together they form a unique fingerprint.

Cite this