High-speed detection of DNA translocation in nanopipettes
Research output: Contribution to journal › Article
Colleges, School and Institutes
- Imperial College London
- 3 Politecnico di Milano Milano, Italy.
- Informazione e Bioingegneria
- Dipartimento di Ingegneria Dell'Informazione, Elettronica e Telecomunicazioni, University of Rome la Sapienza
- Politecnico di Milano
We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.
|Publication status||Published - 11 Mar 2016|