High-speed detection of DNA translocation in nanopipettes

Raquel L. Fraccari, Pietro Ciccarella, Azadeh Bahrami, Marco Carminati, Giorgio Ferrari, Tim Albrecht

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)
290 Downloads (Pure)


We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.
Original languageEnglish
Pages (from-to)7604-7611
Issue number14
Publication statusPublished - 11 Mar 2016


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