Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

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Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production. / Browning, Douglas F; Godfrey, Rita E; Richards, Kirsty L; Robinson, Colin; Busby, Stephen J W.

In: Biochemical Society Transactions, Vol. 47, No. 2, 30.04.2019, p. 755-763.

Research output: Contribution to journalReview articlepeer-review

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@article{e4bce5ce090d4aab840a1d2623d325f8,
title = "Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production",
abstract = "The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.",
keywords = "Escherichia coli/genetics, Lac Operon/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Repressor Proteins/genetics, Transcription, Genetic/genetics",
author = "Browning, {Douglas F} and Godfrey, {Rita E} and Richards, {Kirsty L} and Colin Robinson and Busby, {Stephen J W}",
note = "{\textcopyright} 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.",
year = "2019",
month = apr,
day = "30",
doi = "10.1042/BST20190059",
language = "English",
volume = "47",
pages = "755--763",
journal = "Biochemical Society Transactions",
issn = "1470-8752",
publisher = "Portland Press",
number = "2",

}

RIS

TY - JOUR

T1 - Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

AU - Browning, Douglas F

AU - Godfrey, Rita E

AU - Richards, Kirsty L

AU - Robinson, Colin

AU - Busby, Stephen J W

N1 - © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

PY - 2019/4/30

Y1 - 2019/4/30

N2 - The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

AB - The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

KW - Escherichia coli/genetics

KW - Lac Operon/genetics

KW - Promoter Regions, Genetic/genetics

KW - Recombinant Proteins/genetics

KW - Repressor Proteins/genetics

KW - Transcription, Genetic/genetics

UR - http://www.scopus.com/inward/record.url?scp=85065030944&partnerID=8YFLogxK

U2 - 10.1042/BST20190059

DO - 10.1042/BST20190059

M3 - Review article

C2 - 30971435

VL - 47

SP - 755

EP - 763

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 1470-8752

IS - 2

ER -