Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Douglas F Browning, Rita E Godfrey, Kirsty L Richards, Colin Robinson, Stephen J W Busby

Research output: Contribution to journalReview articlepeer-review

9 Citations (Scopus)
377 Downloads (Pure)

Abstract

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

Original languageEnglish
Pages (from-to)755-763
Number of pages9
JournalBiochemical Society Transactions
Volume47
Issue number2
Early online date10 Apr 2019
DOIs
Publication statusPublished - 30 Apr 2019

Bibliographical note

© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

Keywords

  • Escherichia coli/genetics
  • Lac Operon/genetics
  • Promoter Regions, Genetic/genetics
  • Recombinant Proteins/genetics
  • Repressor Proteins/genetics
  • Transcription, Genetic/genetics

ASJC Scopus subject areas

  • Biochemistry

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