Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Douglas F Browning; Rita E Godfrey; Kirsty L Richards; Colin Robinson; Stephen J W Busby

doi:10.1042/BST20190059

Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Douglas F Browning, Rita E Godfrey, Kirsty L Richards, Colin Robinson, Stephen J W Busby

Biosciences

Research output: Contribution to journal › Review article › peer-review

9 Citations (Scopus)

762 Downloads (Pure)

Abstract

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

Original language	English
Pages (from-to)	755-763
Number of pages	9
Journal	Biochemical Society Transactions
Volume	47
Issue number	2
Early online date	10 Apr 2019
DOIs	https://doi.org/10.1042/BST20190059
Publication status	Published - 30 Apr 2019

Bibliographical note

Keywords

Escherichia coli/genetics
Lac Operon/genetics
Promoter Regions, Genetic/genetics
Recombinant Proteins/genetics
Repressor Proteins/genetics
Transcription, Genetic/genetics

ASJC Scopus subject areas

Biochemistry

Access to Document

10.1042/BST20190059Licence: None: All rights reserved

Douglas_F_Browning_et_al_Exploitation_of_the_Escherichia_coli_lac_operon_promoter_Biochemical_Society_Transactions_2019
Checked for eligibility: 12/04/2019 This document is the accepted manuscript version of a published work that appeared in final form in Biochemical Society Transactions. The final version of record can be found at: https://doi.org/10.1042/BST20190059
Accepted author manuscript, 951 KBLicence: None: All rights reserved

Cite this

@article{e4bce5ce090d4aab840a1d2623d325f8,

title = "Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production",

abstract = "The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.",

keywords = "Escherichia coli/genetics, Lac Operon/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Repressor Proteins/genetics, Transcription, Genetic/genetics",

author = "Browning, {Douglas F} and Godfrey, {Rita E} and Richards, {Kirsty L} and Colin Robinson and Busby, {Stephen J W}",

note = "{\textcopyright} 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.",

year = "2019",

month = apr,

day = "30",

doi = "10.1042/BST20190059",

language = "English",

volume = "47",

pages = "755--763",

journal = "Biochemical Society Transactions",

issn = "1470-8752",

publisher = "Portland Press",

number = "2",

}

TY - JOUR

T1 - Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

AU - Browning, Douglas F

AU - Godfrey, Rita E

AU - Richards, Kirsty L

AU - Robinson, Colin

AU - Busby, Stephen J W

PY - 2019/4/30

Y1 - 2019/4/30

N2 - The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

AB - The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

KW - Escherichia coli/genetics

KW - Lac Operon/genetics

KW - Promoter Regions, Genetic/genetics

KW - Recombinant Proteins/genetics

KW - Repressor Proteins/genetics

KW - Transcription, Genetic/genetics

UR - http://www.scopus.com/inward/record.url?scp=85065030944&partnerID=8YFLogxK

U2 - 10.1042/BST20190059

DO - 10.1042/BST20190059

M3 - Review article

C2 - 30971435

SN - 1470-8752

VL - 47

SP - 755

EP - 763

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - 2

ER -

Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this