Dissociation techniques in mass spectrometry-based proteomics

Research output: Contribution to journalReview article

Colleges, School and Institutes

Abstract

The field of proteomics, the large-scale analysis of proteins, has undergone a huge expansion over the past decade. Mass spectrometry-based proteomics relies on the dissociation of peptide and/or protein ions to provide information on primary sequence and sites of post-translational modifications. Fragmentation techniques include collision-induced dissociation, electron capture dissociation and electron transfer dissociation. Here, we describe each of these techniques and their use in proteomics. The principles, advantages, limitations, and applications are discussed.

Details

Original languageEnglish
Pages (from-to)3419-3429
Number of pages11
JournalThe Analyst
Volume136
Issue number17
Publication statusPublished - 1 Jan 2011