Abstract
The field of proteomics, the large-scale analysis of proteins, has undergone a huge expansion over the past decade. Mass spectrometry-based proteomics relies on the dissociation of peptide and/or protein ions to provide information on primary sequence and sites of post-translational modifications. Fragmentation techniques include collision-induced dissociation, electron capture dissociation and electron transfer dissociation. Here, we describe each of these techniques and their use in proteomics. The principles, advantages, limitations, and applications are discussed.
Original language | English |
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Pages (from-to) | 3419-3429 |
Number of pages | 11 |
Journal | The Analyst |
Volume | 136 |
Issue number | 17 |
DOIs | |
Publication status | Published - 1 Jan 2011 |