Backscattered Electron Imaging and Electron Backscattered Diffraction in the Study of Bacterial Attachment to Titanium Alloy Structure

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@article{dfd3056ddf43406ea92286a3b41af0dc,
title = "Backscattered Electron Imaging and Electron Backscattered Diffraction in the Study of Bacterial Attachment to Titanium Alloy Structure",
abstract = "The application of secondary electron (SE) imaging, backscattered electron imaging (BSE) and electron backscattered diffraction (EBSD) was investigated in this work to study the bacterial adhesion and proliferation on a commercially pure titanium (cp Ti) and a Ti6Al4V alloy (Ti 64) with respect to substrate microstructure and chemical composition. Adherence of Gram-positive Staphylococcus epidermidis 11047 and Streptococcus sanguinis GW2, and Gram-negative Serratia sp. NCIMB 40259 and Escherichia coli 10418 was compared on cp Ti, Ti 64, pure aluminium (Al) and vanadium (V). The substrate microstructure and the bacterial distribution on these metals were characterised using SE, BSE and EBSD imaging. It was observed that titanium alloy-phase structure, grain boundaries and grain orientation did not influence bacterial adherence or proliferation at microscale. Adherence of all four strains was similar on cp Ti and Ti 64 surfaces whilst inhibited on pure Al. This work establishes a nondestructive and straight-forward statistical method to analyse the relationship between microbial distribution and metal alloy structure.",
keywords = "electron microscopy , bacteria adhesion , grain boundary , grain orientation , chemical composition , microstructure",
author = "Anqi Wang and Ian Jones and Junfa Mei and Tse, {Yau Yau} and Gabriel Landini and Yue Li and Linnan Ke and Yuanli Huang and Li Liu and Chunren Wang and Rachel Sammons",
year = "2017",
month = oct,
day = "11",
doi = "10.1111/jmi.12649",
language = "English",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley",

}

RIS

TY - JOUR

T1 - Backscattered Electron Imaging and Electron Backscattered Diffraction in the Study of Bacterial Attachment to Titanium Alloy Structure

AU - Wang, Anqi

AU - Jones, Ian

AU - Mei, Junfa

AU - Tse, Yau Yau

AU - Landini, Gabriel

AU - Li, Yue

AU - Ke, Linnan

AU - Huang, Yuanli

AU - Liu, Li

AU - Wang, Chunren

AU - Sammons, Rachel

PY - 2017/10/11

Y1 - 2017/10/11

N2 - The application of secondary electron (SE) imaging, backscattered electron imaging (BSE) and electron backscattered diffraction (EBSD) was investigated in this work to study the bacterial adhesion and proliferation on a commercially pure titanium (cp Ti) and a Ti6Al4V alloy (Ti 64) with respect to substrate microstructure and chemical composition. Adherence of Gram-positive Staphylococcus epidermidis 11047 and Streptococcus sanguinis GW2, and Gram-negative Serratia sp. NCIMB 40259 and Escherichia coli 10418 was compared on cp Ti, Ti 64, pure aluminium (Al) and vanadium (V). The substrate microstructure and the bacterial distribution on these metals were characterised using SE, BSE and EBSD imaging. It was observed that titanium alloy-phase structure, grain boundaries and grain orientation did not influence bacterial adherence or proliferation at microscale. Adherence of all four strains was similar on cp Ti and Ti 64 surfaces whilst inhibited on pure Al. This work establishes a nondestructive and straight-forward statistical method to analyse the relationship between microbial distribution and metal alloy structure.

AB - The application of secondary electron (SE) imaging, backscattered electron imaging (BSE) and electron backscattered diffraction (EBSD) was investigated in this work to study the bacterial adhesion and proliferation on a commercially pure titanium (cp Ti) and a Ti6Al4V alloy (Ti 64) with respect to substrate microstructure and chemical composition. Adherence of Gram-positive Staphylococcus epidermidis 11047 and Streptococcus sanguinis GW2, and Gram-negative Serratia sp. NCIMB 40259 and Escherichia coli 10418 was compared on cp Ti, Ti 64, pure aluminium (Al) and vanadium (V). The substrate microstructure and the bacterial distribution on these metals were characterised using SE, BSE and EBSD imaging. It was observed that titanium alloy-phase structure, grain boundaries and grain orientation did not influence bacterial adherence or proliferation at microscale. Adherence of all four strains was similar on cp Ti and Ti 64 surfaces whilst inhibited on pure Al. This work establishes a nondestructive and straight-forward statistical method to analyse the relationship between microbial distribution and metal alloy structure.

KW - electron microscopy

KW - bacteria adhesion

KW - grain boundary

KW - grain orientation

KW - chemical composition

KW - microstructure

U2 - 10.1111/jmi.12649

DO - 10.1111/jmi.12649

M3 - Article

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

ER -