Automatic exposure control
and estimation of effective system noise
in diffuse fluorescence tomography

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

A diffuse fluorescence tomography system, based upon time-correlated single photon counting, is presented with an automated algorithm to allow dynamic range variation through exposure control. This automated exposure control allows the upper and lower detection levels of fluorophore to be extended by an order of magnitude beyond the previously published performance and benefits in a slight decrease in system effective noise. The effective noise level is used as a metric to characterize the system performance, integrating both model-mismatch and calibration bias errors into a single parameter. This effective error is near 7% of the reconstructed fluorescent yield value, when imaging in just few minutes. Quantifying protoporphyrin IX concentrations down to 50 ng/ml is possible, for tumor-sized regions. This fluorophore has very low fluorescence yield, but high biological relevance for tumor imaging, given that it is produced in the mitochondria, and upregulated in many tumor types. (C) 2009 Optical Society of America

Details

Original languageEnglish
Pages (from-to)23272-23283
Number of pages12
JournalOptics Express
Volume17
Issue number25
Publication statusPublished - 1 Jan 2009

Keywords

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