Adhesion of tumor cells to matrices and endothelium
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.
|Number of pages||19|
|Journal||Methods in molecular biology|
|Publication status||Published - 2014|
- Animals, Cell Adhesion, Cell Culture Techniques, Cell Line, Tumor, Collagen, Endothelial Cells, Endothelium, Extracellular Matrix, Gels, Humans, Neoplasms, Rats, Rheology