Adhesion of tumor cells to matrices and endothelium

Clara M Yates; Helen M McGettrick; Gerard B Nash; G Ed Rainger

doi:10.1007/978-1-4614-8244-4_5

Adhesion of tumor cells to matrices and endothelium

Clara M Yates, Helen M McGettrick, Gerard B Nash, G Ed Rainger

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.

Original language	English
Pages (from-to)	57-75
Number of pages	19
Journal	Methods in molecular biology
Volume	1070
DOIs	https://doi.org/10.1007/978-1-4614-8244-4_5
Publication status	Published - 2014

Keywords

Animals
Cell Adhesion
Cell Culture Techniques
Cell Line, Tumor
Collagen
Endothelial Cells
Endothelium
Extracellular Matrix
Gels
Humans
Neoplasms
Rats
Rheology

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10.1007/978-1-4614-8244-4_5

MRC Centre For Immune Regulation (Linked to DCDF.RRAK10540) (Linked to 14810 & 14835)
Jenkinson, E.
Medical Research Council
3/08/09 → 30/09/17
Project: Research Councils
The Ability of Fatty Acids to Modulate the Inflammatory Effects of Macrophage/Monocytes on Endothelial Cells
Rainger, E. & Nash, G.
Biotechnology & Biological Sciences Research Council
1/04/04 → 31/05/07
Project: Research Councils

Cite this

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title = "Adhesion of tumor cells to matrices and endothelium",

abstract = "Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.",

keywords = "Animals, Cell Adhesion, Cell Culture Techniques, Cell Line, Tumor, Collagen, Endothelial Cells, Endothelium, Extracellular Matrix, Gels, Humans, Neoplasms, Rats, Rheology",

author = "Yates, {Clara M} and McGettrick, {Helen M} and Nash, {Gerard B} and Rainger, {G Ed}",

year = "2014",

doi = "10.1007/978-1-4614-8244-4_5",

language = "English",

volume = "1070",

pages = "57--75",

journal = "Methods in molecular biology",

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TY - JOUR

T1 - Adhesion of tumor cells to matrices and endothelium

AU - Yates, Clara M

AU - McGettrick, Helen M

AU - Nash, Gerard B

AU - Rainger, G Ed

PY - 2014

Y1 - 2014

N2 - Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.

AB - Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.

KW - Animals

KW - Cell Adhesion

KW - Cell Culture Techniques

KW - Cell Line, Tumor

KW - Collagen

KW - Endothelial Cells

KW - Endothelium

KW - Extracellular Matrix

KW - Gels

KW - Humans

KW - Neoplasms

KW - Rats

KW - Rheology

U2 - 10.1007/978-1-4614-8244-4_5

DO - 10.1007/978-1-4614-8244-4_5

M3 - Article

C2 - 24092432

SN - 1064-3745

VL - 1070

SP - 57

EP - 75

JO - Methods in molecular biology

JF - Methods in molecular biology

ER -

Adhesion of tumor cells to matrices and endothelium

Abstract

Keywords

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Projects

MRC Centre For Immune Regulation (Linked to DCDF.RRAK10540) (Linked to 14810 & 14835)

The Ability of Fatty Acids to Modulate the Inflammatory Effects of Macrophage/Monocytes on Endothelial Cells

Cite this