A simple assay method for determination of the specific radioactivity of the gamma-phosphate group of 32P-labelled ATP.

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Authors

Colleges, School and Institutes

Abstract

A simple and rapid assay method is described for determining the specific radioactivity of the gamma-phosphate group of 32P-labelled ATP. Labelled ATP is incubated with cyclic AMP, cyclic AMP-dependent protein kinase and histone H2A under conditions leading to maximum phosphorylation of the histone. The specific radioactivity of the gamma-phosphate group of the 32P-labelled ATP is calculated from the total amount of [32P]Pi incorporated into a standard amount of histone. This assay method uses inexpensive commercially available materials, and it yields an accurate specific radioactivity with as little as 0.25 nmol of 32P-labelled ATP.

Details

Original languageEnglish
Pages (from-to)717-720
Number of pages4
JournalThe Biochemical journal
Volume210
Issue number3
Publication statusPublished - 1 Jan 1983