Abstract
A simple and rapid assay method is described for determining the specific radioactivity of the gamma-phosphate group of 32P-labelled ATP. Labelled ATP is incubated with cyclic AMP, cyclic AMP-dependent protein kinase and histone H2A under conditions leading to maximum phosphorylation of the histone. The specific radioactivity of the gamma-phosphate group of the 32P-labelled ATP is calculated from the total amount of [32P]Pi incorporated into a standard amount of histone. This assay method uses inexpensive commercially available materials, and it yields an accurate specific radioactivity with as little as 0.25 nmol of 32P-labelled ATP.
Original language | English |
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Pages (from-to) | 717-720 |
Number of pages | 4 |
Journal | The Biochemical journal |
Volume | 210 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Jan 1983 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology