A RIPE3b1-like factor binds to a novel site in the human insulin promoter in a redox-dependent manner

Research output: Contribution to journalArticle

Authors

Abstract

In the human insulin gene, a regulatory sequence upstream of the transcription start site at -229 to -258 (the E2 element) binds a ubiquitous factor USF. The present study led to the identification of a second factor, D0, that binds to an adjacent upstream site, the C2 element, that has previously not been described. The results demonstrate that D0 exhibits similar properties to RIPE3b1, a factor shown to be an important determinant of insulin gene beta-cell-specific expression. Binding of D0 to the C2 element was abolished by the oxidising agent diamide, and the alkylating agent N-ethylmaleimide. The results indicate that expression of the insulin gene may be regulated by a redox-dependent pathway involving RIPE3b1 or a RIPE3b1-like factor.

Details

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalFEBS Letters
Volume418
Issue number1-2
Publication statusPublished - 24 Nov 1997

Keywords

  • Animals, Base Sequence, Binding Sites, DNA-Binding Proteins, Humans, Insulin, Islets of Langerhans, Oxidation-Reduction, Promoter Regions, Genetic, Rats, Regulatory Sequences, Nucleic Acid, Sequence Alignment, Transcription, Genetic