Abstract
In the human insulin gene, a regulatory sequence upstream of the transcription start site at -229 to -258 (the E2 element) binds a ubiquitous factor USF. The present study led to the identification of a second factor, D0, that binds to an adjacent upstream site, the C2 element, that has previously not been described. The results demonstrate that D0 exhibits similar properties to RIPE3b1, a factor shown to be an important determinant of insulin gene beta-cell-specific expression. Binding of D0 to the C2 element was abolished by the oxidising agent diamide, and the alkylating agent N-ethylmaleimide. The results indicate that expression of the insulin gene may be regulated by a redox-dependent pathway involving RIPE3b1 or a RIPE3b1-like factor.
Original language | English |
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Pages (from-to) | 68-72 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 418 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 24 Nov 1997 |
Keywords
- Animals
- Base Sequence
- Binding Sites
- DNA-Binding Proteins
- Humans
- Insulin
- Islets of Langerhans
- Oxidation-Reduction
- Promoter Regions, Genetic
- Rats
- Regulatory Sequences, Nucleic Acid
- Sequence Alignment
- Transcription, Genetic