A RIPE3b1-like factor binds to a novel site in the human insulin promoter in a redox-dependent manner

Martin Read, M R Masson, K Docherty

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    In the human insulin gene, a regulatory sequence upstream of the transcription start site at -229 to -258 (the E2 element) binds a ubiquitous factor USF. The present study led to the identification of a second factor, D0, that binds to an adjacent upstream site, the C2 element, that has previously not been described. The results demonstrate that D0 exhibits similar properties to RIPE3b1, a factor shown to be an important determinant of insulin gene beta-cell-specific expression. Binding of D0 to the C2 element was abolished by the oxidising agent diamide, and the alkylating agent N-ethylmaleimide. The results indicate that expression of the insulin gene may be regulated by a redox-dependent pathway involving RIPE3b1 or a RIPE3b1-like factor.

    Original languageEnglish
    Pages (from-to)68-72
    Number of pages5
    JournalFEBS Letters
    Volume418
    Issue number1-2
    DOIs
    Publication statusPublished - 24 Nov 1997

    Keywords

    • Animals
    • Base Sequence
    • Binding Sites
    • DNA-Binding Proteins
    • Humans
    • Insulin
    • Islets of Langerhans
    • Oxidation-Reduction
    • Promoter Regions, Genetic
    • Rats
    • Regulatory Sequences, Nucleic Acid
    • Sequence Alignment
    • Transcription, Genetic

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