USP7/HAUSP stimulates repair of oxidative DNA lesions

Svetlana V Khoronenkova; Irina I Dianova; Jason L Parsons; Grigory L Dianov

doi:10.1093/nar/gkq1210

USP7/HAUSP stimulates repair of oxidative DNA lesions

Svetlana V Khoronenkova, Irina I Dianova, Jason L Parsons, Grigory L Dianov

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

USP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiquitin ligases involved in histone ubiquitylation. Here, we provide new evidence that USP7 modulated chromatin remodelling is important for base excision repair of oxidative lesions. We show that transient USP7 siRNA knockdown did not change the levels or activity of base excision repair enzymes, but significantly reduced chromatin DNA accessibility and consequently the rate of repair of oxidative lesions.

Original language	English
Pages (from-to)	2604-9
Number of pages	6
Journal	Nucleic Acids Research
Volume	39
Issue number	7
DOIs	https://doi.org/10.1093/nar/gkq1210
Publication status	Published - Apr 2011

Keywords

Chromatin/metabolism
Chromatin Assembly and Disassembly
DNA Damage
DNA Repair
DNA Repair Enzymes/metabolism
HeLa Cells
Humans
Oxidation-Reduction
Proto-Oncogene Proteins c-mdm2/metabolism
RNA Interference
Ubiquitin Thiolesterase/antagonists & inhibitors
Ubiquitin-Specific Peptidase 7

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10.1093/nar/gkq1210

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title = "USP7/HAUSP stimulates repair of oxidative DNA lesions",

abstract = "USP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiquitin ligases involved in histone ubiquitylation. Here, we provide new evidence that USP7 modulated chromatin remodelling is important for base excision repair of oxidative lesions. We show that transient USP7 siRNA knockdown did not change the levels or activity of base excision repair enzymes, but significantly reduced chromatin DNA accessibility and consequently the rate of repair of oxidative lesions.",

keywords = "Chromatin/metabolism, Chromatin Assembly and Disassembly, DNA Damage, DNA Repair, DNA Repair Enzymes/metabolism, HeLa Cells, Humans, Oxidation-Reduction, Proto-Oncogene Proteins c-mdm2/metabolism, RNA Interference, Ubiquitin Thiolesterase/antagonists & inhibitors, Ubiquitin-Specific Peptidase 7",

author = "Khoronenkova, {Svetlana V} and Dianova, {Irina I} and Parsons, {Jason L} and Dianov, {Grigory L}",

year = "2011",

month = apr,

doi = "10.1093/nar/gkq1210",

language = "English",

volume = "39",

pages = "2604--9",

journal = "Nucleic Acids Research",

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TY - JOUR

T1 - USP7/HAUSP stimulates repair of oxidative DNA lesions

AU - Khoronenkova, Svetlana V

AU - Dianova, Irina I

AU - Parsons, Jason L

AU - Dianov, Grigory L

PY - 2011/4

Y1 - 2011/4

N2 - USP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiquitin ligases involved in histone ubiquitylation. Here, we provide new evidence that USP7 modulated chromatin remodelling is important for base excision repair of oxidative lesions. We show that transient USP7 siRNA knockdown did not change the levels or activity of base excision repair enzymes, but significantly reduced chromatin DNA accessibility and consequently the rate of repair of oxidative lesions.

AB - USP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiquitin ligases involved in histone ubiquitylation. Here, we provide new evidence that USP7 modulated chromatin remodelling is important for base excision repair of oxidative lesions. We show that transient USP7 siRNA knockdown did not change the levels or activity of base excision repair enzymes, but significantly reduced chromatin DNA accessibility and consequently the rate of repair of oxidative lesions.

KW - Chromatin/metabolism

KW - Chromatin Assembly and Disassembly

KW - DNA Damage

KW - DNA Repair

KW - DNA Repair Enzymes/metabolism

KW - HeLa Cells

KW - Humans

KW - Oxidation-Reduction

KW - Proto-Oncogene Proteins c-mdm2/metabolism

KW - RNA Interference

KW - Ubiquitin Thiolesterase/antagonists & inhibitors

KW - Ubiquitin-Specific Peptidase 7

U2 - 10.1093/nar/gkq1210

DO - 10.1093/nar/gkq1210

M3 - Article

C2 - 21138959

SN - 0305-1048

VL - 39

SP - 2604

EP - 2609

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 7

ER -

USP7/HAUSP stimulates repair of oxidative DNA lesions

Abstract

Keywords

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