Projects per year
Prospero is required in dividing longitudinal glia (LG) during axon guidance; initially to enable glial division in response to neuronal contact, and subsequently to maintain glial precursors in a quiescent state with mitotic potential. Only Prospero-positive LG respond to neuronal ablation by over-proliferating, mimicking a glial-repair response. Prospero is distributed unequally through the progeny cells of the longitudinal glioblast lineage. Just before axon contact the concentration of Prospero is higher in two of the four progeny cells, and after axon guidance Prospero is present only in six out of tell progeny LG. Here we ask how Prospero is distributed unequally in these two distinct phases, We show, that before neuronal contact, longitudinal glioblasts undergo invaginating divisions, perpendicular to the ectodermal layer. Miranda is required to segregate Prospero asymmetrically tip to the four glial-progeny stage. After neuronal contact, Prospero is present in only the LG that activate Notch signalling in response to Serrate provided by commissural axons, and Numb is restricted to the glia that do not contain Prospero. As a result of this dual regulation of Prospero deployment, glia are coupled to the formation and maintenance of axonal trajectories.
- axon guidance
- neuron-glia interaction
FingerprintDive into the research topics of 'Two distinct mechanisms segregated Prospero in the longitudinal glia underlying the timing of interactions with axons'. Together they form a unique fingerprint.
- 1 Finished
Functional Genetics of Trophic Support in Neuron-Glia Interactions in the Central Nervous System of Drosphila
21/04/03 → 20/08/08
Project: Research Councils