Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic culture

Adam F Cunningham; Peter R Ashton; Claire L Spreadbury; David A Lammas; Rachel Craddock; Christopher W Wharton; Paul R Wheeler

doi:10.1016/j.femsle.2004.04.036

Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic culture

Adam F Cunningham, Peter R Ashton, Claire L Spreadbury, David A Lammas, Rachel Craddock, Christopher W Wharton, Paul R Wheeler

Research output: Contribution to journal › Article › peer-review

Abstract

Many cases of tuberculosis result from reactivation of previously acquired latent infections. Models to study such persister forms often involve gradual depletion of oxygen during culture as poor aeration is a characteristic of non-progressive TB granulomas. Anaerobically cultured bacilli develop a thickened outer-most cell wall layer. Here, we analyzed this layer from anaerobically cultured Mycobacterium tuberculosis and Mycobacterium bovis BCG. By six weeks of anaerobiosis a pigment was detected at levels > 60-fold higher in anaerobic than aerobic bacilli. This pigment was responsible for the electron-dense appearance of the thickened cell wall layer and gave an electrospray mass spectrometry peak at 409 Da (M+Na)+ or (M+H)+. We termed this pigment APP1, anaerobically produced pigment 1, the first pigment identified in M. tuberculosis.

Original language	English
Pages (from-to)	191-8
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	235
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2004.04.036
Publication status	Published - 1 Jun 2004

Keywords

Anaerobiosis
Cell Wall/chemistry
Ethanol/chemistry
Molecular Weight
Mycobacterium bovis/chemistry
Mycobacterium tuberculosis/chemistry
Pigments, Biological/analysis
Spectrometry, Mass, Electrospray Ionization
Time Factors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.femsle.2004.04.036

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title = "Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic culture",

abstract = "Many cases of tuberculosis result from reactivation of previously acquired latent infections. Models to study such persister forms often involve gradual depletion of oxygen during culture as poor aeration is a characteristic of non-progressive TB granulomas. Anaerobically cultured bacilli develop a thickened outer-most cell wall layer. Here, we analyzed this layer from anaerobically cultured Mycobacterium tuberculosis and Mycobacterium bovis BCG. By six weeks of anaerobiosis a pigment was detected at levels > 60-fold higher in anaerobic than aerobic bacilli. This pigment was responsible for the electron-dense appearance of the thickened cell wall layer and gave an electrospray mass spectrometry peak at 409 Da (M+Na)+ or (M+H)+. We termed this pigment APP1, anaerobically produced pigment 1, the first pigment identified in M. tuberculosis.",

keywords = "Anaerobiosis, Cell Wall/chemistry, Ethanol/chemistry, Molecular Weight, Mycobacterium bovis/chemistry, Mycobacterium tuberculosis/chemistry, Pigments, Biological/analysis, Spectrometry, Mass, Electrospray Ionization, Time Factors",

author = "Cunningham, {Adam F} and Ashton, {Peter R} and Spreadbury, {Claire L} and Lammas, {David A} and Rachel Craddock and Wharton, {Christopher W} and Wheeler, {Paul R}",

year = "2004",

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doi = "10.1016/j.femsle.2004.04.036",

language = "English",

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TY - JOUR

T1 - Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic culture

AU - Cunningham, Adam F

AU - Ashton, Peter R

AU - Spreadbury, Claire L

AU - Lammas, David A

AU - Craddock, Rachel

AU - Wharton, Christopher W

AU - Wheeler, Paul R

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Many cases of tuberculosis result from reactivation of previously acquired latent infections. Models to study such persister forms often involve gradual depletion of oxygen during culture as poor aeration is a characteristic of non-progressive TB granulomas. Anaerobically cultured bacilli develop a thickened outer-most cell wall layer. Here, we analyzed this layer from anaerobically cultured Mycobacterium tuberculosis and Mycobacterium bovis BCG. By six weeks of anaerobiosis a pigment was detected at levels > 60-fold higher in anaerobic than aerobic bacilli. This pigment was responsible for the electron-dense appearance of the thickened cell wall layer and gave an electrospray mass spectrometry peak at 409 Da (M+Na)+ or (M+H)+. We termed this pigment APP1, anaerobically produced pigment 1, the first pigment identified in M. tuberculosis.

AB - Many cases of tuberculosis result from reactivation of previously acquired latent infections. Models to study such persister forms often involve gradual depletion of oxygen during culture as poor aeration is a characteristic of non-progressive TB granulomas. Anaerobically cultured bacilli develop a thickened outer-most cell wall layer. Here, we analyzed this layer from anaerobically cultured Mycobacterium tuberculosis and Mycobacterium bovis BCG. By six weeks of anaerobiosis a pigment was detected at levels > 60-fold higher in anaerobic than aerobic bacilli. This pigment was responsible for the electron-dense appearance of the thickened cell wall layer and gave an electrospray mass spectrometry peak at 409 Da (M+Na)+ or (M+H)+. We termed this pigment APP1, anaerobically produced pigment 1, the first pigment identified in M. tuberculosis.

KW - Anaerobiosis

KW - Cell Wall/chemistry

KW - Ethanol/chemistry

KW - Molecular Weight

KW - Mycobacterium bovis/chemistry

KW - Mycobacterium tuberculosis/chemistry

KW - Pigments, Biological/analysis

KW - Spectrometry, Mass, Electrospray Ionization

KW - Time Factors

U2 - 10.1016/j.femsle.2004.04.036

DO - 10.1016/j.femsle.2004.04.036

M3 - Article

C2 - 15158281

SN - 0378-1097

VL - 235

SP - 191

EP - 198

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic culture

Abstract

Keywords

UN SDGs

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