Tribological Characteristics of Human Vascular Smooth Muscle Cells: The Implication of Disease State on Friction

Emily R. Clark; Karen Hemmings; Silvia Greco; Anne Neville; Karen E. Porter; Michael G. Bryant

doi:10.1016/j.biotri.2020.100122

Tribological Characteristics of Human Vascular Smooth Muscle Cells: The Implication of Disease State on Friction

Emily R. Clark, Karen Hemmings, Silvia Greco, Anne Neville, Karen E. Porter, Michael G. Bryant^*

^*Corresponding author for this work

Engineering and Physical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to establish a simple tribological model to assess the frictional properties of non-diabetic (ND) and Type 2 diabetic (T2DM) patients with the view to better understand the interfacial processes off icing in-vivo during angioplasty. Human primary smooth muscle cell (SMC) monolayers from non-diabetic (ND) and Type 2 diabetic (T2DM) patients were isolated and cultured. The coefficient of friction of ND and T2DM SMC monolayers was measured using a micro-tribometer set-up at normal loads (F_n) of 0.4 and 0.8 mN. The coefficient of friction was dependent on load and disease state. The cycle average coefficient of friction of patient grouped SMCs was μ = 0.107 ± 0.03 and 0.22 ± 0.01 for ND and T2DM respectively. Within the ND group, the coefficient of friction was seen to be patient specific, with the coefficient of friction varying significantly from μ = 0.03 ± 0.03 to 0.185 ± 0.07. Results show that disease state will affect the frictional properties of SMCs. In turn the disease state may also influence the SMCs susceptibly to tribologically induced inflammation.

Original language	English
Article number	100122
Journal	Biotribology
Volume	22
DOIs	https://doi.org/10.1016/j.biotri.2020.100122
Publication status	Published - Jun 2020

Bibliographical note

Funding Information:
This work was supported by the Engineering and Physical Sciences Research Council ( EP/P009662/1 ).

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

Cells
Diabetes
Friction
ISR
Smooth muscle cells

ASJC Scopus subject areas

Biomaterials
Surfaces, Coatings and Films

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biotri.2020.100122

Cite this

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title = "Tribological Characteristics of Human Vascular Smooth Muscle Cells: The Implication of Disease State on Friction",

abstract = "The aim of this study was to establish a simple tribological model to assess the frictional properties of non-diabetic (ND) and Type 2 diabetic (T2DM) patients with the view to better understand the interfacial processes off icing in-vivo during angioplasty. Human primary smooth muscle cell (SMC) monolayers from non-diabetic (ND) and Type 2 diabetic (T2DM) patients were isolated and cultured. The coefficient of friction of ND and T2DM SMC monolayers was measured using a micro-tribometer set-up at normal loads (Fn) of 0.4 and 0.8 mN. The coefficient of friction was dependent on load and disease state. The cycle average coefficient of friction of patient grouped SMCs was μ = 0.107 ± 0.03 and 0.22 ± 0.01 for ND and T2DM respectively. Within the ND group, the coefficient of friction was seen to be patient specific, with the coefficient of friction varying significantly from μ = 0.03 ± 0.03 to 0.185 ± 0.07. Results show that disease state will affect the frictional properties of SMCs. In turn the disease state may also influence the SMCs susceptibly to tribologically induced inflammation.",

keywords = "Cells, Diabetes, Friction, ISR, Smooth muscle cells",

author = "Clark, {Emily R.} and Karen Hemmings and Silvia Greco and Anne Neville and Porter, {Karen E.} and Bryant, {Michael G.}",

note = "Funding Information: This work was supported by the Engineering and Physical Sciences Research Council ( EP/P009662/1 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

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doi = "10.1016/j.biotri.2020.100122",

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AU - Hemmings, Karen

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AU - Neville, Anne

AU - Porter, Karen E.

AU - Bryant, Michael G.

PY - 2020/6

Y1 - 2020/6

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AB - The aim of this study was to establish a simple tribological model to assess the frictional properties of non-diabetic (ND) and Type 2 diabetic (T2DM) patients with the view to better understand the interfacial processes off icing in-vivo during angioplasty. Human primary smooth muscle cell (SMC) monolayers from non-diabetic (ND) and Type 2 diabetic (T2DM) patients were isolated and cultured. The coefficient of friction of ND and T2DM SMC monolayers was measured using a micro-tribometer set-up at normal loads (Fn) of 0.4 and 0.8 mN. The coefficient of friction was dependent on load and disease state. The cycle average coefficient of friction of patient grouped SMCs was μ = 0.107 ± 0.03 and 0.22 ± 0.01 for ND and T2DM respectively. Within the ND group, the coefficient of friction was seen to be patient specific, with the coefficient of friction varying significantly from μ = 0.03 ± 0.03 to 0.185 ± 0.07. Results show that disease state will affect the frictional properties of SMCs. In turn the disease state may also influence the SMCs susceptibly to tribologically induced inflammation.

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Tribological Characteristics of Human Vascular Smooth Muscle Cells: The Implication of Disease State on Friction

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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