Transcription factor IID parks and drives preinitiation complexes at sharp or broad promoters

Andrea Bernardini; Camille Hollinger; Damaris Willgenss; Ferenc Müller; Didier Devys; László Tora

doi:10.1016/j.tibs.2023.07.009

Transcription factor IID parks and drives preinitiation complexes at sharp or broad promoters

Andrea Bernardini, Camille Hollinger, Damaris Willgenss, Ferenc Müller, Didier Devys^*, László Tora^*

^*Corresponding author for this work

Cancer and Genomic Sciences

Research output: Contribution to journal › Review article › peer-review

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Abstract

Core promoters are sites where transcriptional regulatory inputs of a gene are integrated to direct the assembly of the preinitiation complex (PIC) and RNA polymerase II (Pol II) transcription output. Until now, core promoter functions have been investigated by distinct methods, including Pol II transcription initiation site mappings and structural characterization of PICs on distinct promoters. Here, we bring together these previously unconnected observations and hypothesize how, on metazoan TATA promoters, the precisely structured building up of transcription factor (TF) IID-based PICs results in sharp transcription start site (TSS) selection; or, in contrast, how the less strictly controlled positioning of the TATA-less promoter DNA relative to TFIID-core PIC components results in alternative broad TSS selections by Pol II.

Original language	English
Pages (from-to)	839-848
Number of pages	10
Journal	Trends in Biochemical Sciences
Volume	48
Issue number	10
Early online date	12 Aug 2023
DOIs	https://doi.org/10.1016/j.tibs.2023.07.009
Publication status	Published - Oct 2023

Bibliographical note

Funding Information:
We are grateful to S. Bour for help in making Figure 2 . This work was financially supported by Agence Nationale de la Recherche (ANR) ANR-19-CE11-0003-02 , ANR-20-CE12-0017-03 , ANR-22-CE11-0013-01_ACT ; Fondation pour la Recherche Médicale (EQU-2021-03012631); NIH MIRA (R35GM139564); and NSF (Award Number: 1933344) grants. A.B. has been supported by the Fondation ARC pour la recherche sur le cancer (ARCPOST-DOC2021080004113). This work, as part of the ITI 2021-2028 program of the University of Strasbourg , was also supported by IdEx Unistra (ANR-10-IDEX-0002), and by SFRI-STRAT’US project (ANR 20-SFRI-0012) and EUR IMCBio (ANR-17-EURE-0023) under the framework of the French Investments for the Future Program.

Publisher Copyright:
© 2023 The Authors

Keywords

core promoter architecture
preinitiation complex
RNA polymerase II
transcription factor IID
transcription initiation
transcription start site selection

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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Cite this

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title = "Transcription factor IID parks and drives preinitiation complexes at sharp or broad promoters",

abstract = "Core promoters are sites where transcriptional regulatory inputs of a gene are integrated to direct the assembly of the preinitiation complex (PIC) and RNA polymerase II (Pol II) transcription output. Until now, core promoter functions have been investigated by distinct methods, including Pol II transcription initiation site mappings and structural characterization of PICs on distinct promoters. Here, we bring together these previously unconnected observations and hypothesize how, on metazoan TATA promoters, the precisely structured building up of transcription factor (TF) IID-based PICs results in sharp transcription start site (TSS) selection; or, in contrast, how the less strictly controlled positioning of the TATA-less promoter DNA relative to TFIID-core PIC components results in alternative broad TSS selections by Pol II.",

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note = "Funding Information: We are grateful to S. Bour for help in making Figure 2 . This work was financially supported by Agence Nationale de la Recherche (ANR) ANR-19-CE11-0003-02 , ANR-20-CE12-0017-03 , ANR-22-CE11-0013-01_ACT ; Fondation pour la Recherche M{\'e}dicale (EQU-2021-03012631); NIH MIRA (R35GM139564); and NSF (Award Number: 1933344) grants. A.B. has been supported by the Fondation ARC pour la recherche sur le cancer (ARCPOST-DOC2021080004113). This work, as part of the ITI 2021-2028 program of the University of Strasbourg , was also supported by IdEx Unistra (ANR-10-IDEX-0002), and by SFRI-STRAT{\textquoteright}US project (ANR 20-SFRI-0012) and EUR IMCBio (ANR-17-EURE-0023) under the framework of the French Investments for the Future Program. Publisher Copyright: {\textcopyright} 2023 The Authors",

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AU - Devys, Didier

AU - Tora, László

N1 - Funding Information: We are grateful to S. Bour for help in making Figure 2 . This work was financially supported by Agence Nationale de la Recherche (ANR) ANR-19-CE11-0003-02 , ANR-20-CE12-0017-03 , ANR-22-CE11-0013-01_ACT ; Fondation pour la Recherche Médicale (EQU-2021-03012631); NIH MIRA (R35GM139564); and NSF (Award Number: 1933344) grants. A.B. has been supported by the Fondation ARC pour la recherche sur le cancer (ARCPOST-DOC2021080004113). This work, as part of the ITI 2021-2028 program of the University of Strasbourg , was also supported by IdEx Unistra (ANR-10-IDEX-0002), and by SFRI-STRAT’US project (ANR 20-SFRI-0012) and EUR IMCBio (ANR-17-EURE-0023) under the framework of the French Investments for the Future Program. Publisher Copyright: © 2023 The Authors

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N2 - Core promoters are sites where transcriptional regulatory inputs of a gene are integrated to direct the assembly of the preinitiation complex (PIC) and RNA polymerase II (Pol II) transcription output. Until now, core promoter functions have been investigated by distinct methods, including Pol II transcription initiation site mappings and structural characterization of PICs on distinct promoters. Here, we bring together these previously unconnected observations and hypothesize how, on metazoan TATA promoters, the precisely structured building up of transcription factor (TF) IID-based PICs results in sharp transcription start site (TSS) selection; or, in contrast, how the less strictly controlled positioning of the TATA-less promoter DNA relative to TFIID-core PIC components results in alternative broad TSS selections by Pol II.

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Transcription factor IID parks and drives preinitiation complexes at sharp or broad promoters

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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