Trafficking and Function of GPCRs in the Endosomal Compartment

Davide Calebiro, Amod Godbole, Sandra Lyga, Martin J. Lohse

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


New methods based on fluorescently labeled agonists, genetically encoded fluorescent sensors, and advanced microscopy techniques, such as fluorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular trafficking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.

Original languageEnglish
Pages (from-to)197-211
Number of pages15
JournalMethods in molecular biology
Early online date20 Sept 2014
Publication statusPublished - 2015


  • Endocytosis inhibitor
  • Fluorescence resonance energy transfer
  • G protein-coupled receptor
  • Highly inclined thin illumination
  • Thyroid follicle
  • Thyroid stimulating hormone
  • Thyroid stimulating hormone receptor
  • Total internal reflection fluorescence microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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