Toxicogenomic assessment of 6-OH-BDE47-induced developmental toxicity in chicken embryos

Ying Peng, Pu Xia, Junjiang Zhang, Daniel L. Villeneuve, Jiamin Zhang, Zhihao Wang, Si Wei, Hongxia Yu, Xiaowei Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are analogs of PBDEs with hundreds of possible structures and are frequently detected in the environment. However, the in vivo evidence on the toxicity of OH-PBDEs is still very limited. Here, the developmental toxicity of 6-OH-BDE47, a predominant congener of OH-PBDEs detected in the environment, in chicken embryos was assessed using a toxicogenomic approach. Fertilized chicken eggs were dosed via in ovo administration of 0.006 to 0.474 nmol 6-OH-BDE47/g egg followed by 18 days of incubation. Significant embryo lethality (LD50 = 1.940 nmol/g egg) and increased hepatic somatic index (HSI) were caused by 6-OH-BDE47 exposure. The functional enrichment of differentially expressed genes (DEGs) was associated with oxidative phosphorylation, generation of precursor metabolites and energy, and electron transport chains, which suggest that 6- OH-BDE47 exposure may disrupt the embryo development by altering the function of energy production in mitochondria. Moreover, aryl hydrocarbon receptor (AhR)- mediated responses including up-regulation of CYP1A4 were observed in the livers of embryos exposed to 6-OH-BDE47. Overall, this study confirmed the embryo lethality by 6-OH-BDE47 and further improved the mechanistic understanding of OH-PBDEs-caused toxicity. Ecological risk assessment via application of both no-observed-effect level (NOEL) and the sensitive NOTEL (transcriptional NOEL) suggested that OH-PBDEs might cause ecological risk to wild birds.

Original languageEnglish
Pages (from-to)12493-12503
Number of pages11
JournalEnvironmental Science and Technology
Volume50
Issue number22
DOIs
Publication statusPublished - 15 Nov 2016

Bibliographical note

Funding Information:
For support, we thank National Natural Science Foundation of China (grant nos. 21322704 and 21677072) and Jiangsu Province Funds for Distinguished Young Scientists (BK20130015). X.Z. was supported by the Fundamental Research Funds for the Central Universities and the Collaborative Innovation Center for Regional Environmental Quality. Although one of the authors is an employee of the United States Environmental Protection Agency (U.S. EPA), the contents of this manuscript neither represent nor necessarily reflect U.S. EPA policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

Publisher Copyright:
© 2016 American Chemical Society.

ASJC Scopus subject areas

  • General Chemistry
  • Environmental Chemistry

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