Toxicogenomic assessment of 6-OH-BDE47-induced developmental toxicity in chicken embryos

Ying Peng; Pu Xia; Junjiang Zhang; Daniel L. Villeneuve; Jiamin Zhang; Zhihao Wang; Si Wei; Hongxia Yu; Xiaowei Zhang

doi:10.1021/acs.est.6b04467

Toxicogenomic assessment of 6-OH-BDE47-induced developmental toxicity in chicken embryos

Ying Peng, Pu Xia, Junjiang Zhang, Daniel L. Villeneuve, Jiamin Zhang, Zhihao Wang, Si Wei, Hongxia Yu, Xiaowei Zhang^*

^*Corresponding author for this work

Biosciences

Research output: Contribution to journal › Article › peer-review

Abstract

Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are analogs of PBDEs with hundreds of possible structures and are frequently detected in the environment. However, the in vivo evidence on the toxicity of OH-PBDEs is still very limited. Here, the developmental toxicity of 6-OH-BDE47, a predominant congener of OH-PBDEs detected in the environment, in chicken embryos was assessed using a toxicogenomic approach. Fertilized chicken eggs were dosed via in ovo administration of 0.006 to 0.474 nmol 6-OH-BDE47/g egg followed by 18 days of incubation. Significant embryo lethality (LD50 = 1.940 nmol/g egg) and increased hepatic somatic index (HSI) were caused by 6-OH-BDE47 exposure. The functional enrichment of differentially expressed genes (DEGs) was associated with oxidative phosphorylation, generation of precursor metabolites and energy, and electron transport chains, which suggest that 6- OH-BDE47 exposure may disrupt the embryo development by altering the function of energy production in mitochondria. Moreover, aryl hydrocarbon receptor (AhR)- mediated responses including up-regulation of CYP1A4 were observed in the livers of embryos exposed to 6-OH-BDE47. Overall, this study confirmed the embryo lethality by 6-OH-BDE47 and further improved the mechanistic understanding of OH-PBDEs-caused toxicity. Ecological risk assessment via application of both no-observed-effect level (NOEL) and the sensitive NOTEL (transcriptional NOEL) suggested that OH-PBDEs might cause ecological risk to wild birds.

Original language	English
Pages (from-to)	12493-12503
Number of pages	11
Journal	Environmental Science and Technology
Volume	50
Issue number	22
DOIs	https://doi.org/10.1021/acs.est.6b04467
Publication status	Published - 15 Nov 2016

Bibliographical note

Funding Information:
For support, we thank National Natural Science Foundation of China (grant nos. 21322704 and 21677072) and Jiangsu Province Funds for Distinguished Young Scientists (BK20130015). X.Z. was supported by the Fundamental Research Funds for the Central Universities and the Collaborative Innovation Center for Regional Environmental Quality. Although one of the authors is an employee of the United States Environmental Protection Agency (U.S. EPA), the contents of this manuscript neither represent nor necessarily reflect U.S. EPA policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

Publisher Copyright:
© 2016 American Chemical Society.

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry

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10.1021/acs.est.6b04467

Cite this

@article{818b8454091240079e526d48126f5a9c,

title = "Toxicogenomic assessment of 6-OH-BDE47-induced developmental toxicity in chicken embryos",

abstract = "Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are analogs of PBDEs with hundreds of possible structures and are frequently detected in the environment. However, the in vivo evidence on the toxicity of OH-PBDEs is still very limited. Here, the developmental toxicity of 6-OH-BDE47, a predominant congener of OH-PBDEs detected in the environment, in chicken embryos was assessed using a toxicogenomic approach. Fertilized chicken eggs were dosed via in ovo administration of 0.006 to 0.474 nmol 6-OH-BDE47/g egg followed by 18 days of incubation. Significant embryo lethality (LD50 = 1.940 nmol/g egg) and increased hepatic somatic index (HSI) were caused by 6-OH-BDE47 exposure. The functional enrichment of differentially expressed genes (DEGs) was associated with oxidative phosphorylation, generation of precursor metabolites and energy, and electron transport chains, which suggest that 6- OH-BDE47 exposure may disrupt the embryo development by altering the function of energy production in mitochondria. Moreover, aryl hydrocarbon receptor (AhR)- mediated responses including up-regulation of CYP1A4 were observed in the livers of embryos exposed to 6-OH-BDE47. Overall, this study confirmed the embryo lethality by 6-OH-BDE47 and further improved the mechanistic understanding of OH-PBDEs-caused toxicity. Ecological risk assessment via application of both no-observed-effect level (NOEL) and the sensitive NOTEL (transcriptional NOEL) suggested that OH-PBDEs might cause ecological risk to wild birds.",

author = "Ying Peng and Pu Xia and Junjiang Zhang and Villeneuve, {Daniel L.} and Jiamin Zhang and Zhihao Wang and Si Wei and Hongxia Yu and Xiaowei Zhang",

note = "Funding Information: For support, we thank National Natural Science Foundation of China (grant nos. 21322704 and 21677072) and Jiangsu Province Funds for Distinguished Young Scientists (BK20130015). X.Z. was supported by the Fundamental Research Funds for the Central Universities and the Collaborative Innovation Center for Regional Environmental Quality. Although one of the authors is an employee of the United States Environmental Protection Agency (U.S. EPA), the contents of this manuscript neither represent nor necessarily reflect U.S. EPA policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = nov,

day = "15",

doi = "10.1021/acs.est.6b04467",

language = "English",

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T1 - Toxicogenomic assessment of 6-OH-BDE47-induced developmental toxicity in chicken embryos

AU - Peng, Ying

AU - Xia, Pu

AU - Zhang, Junjiang

AU - Villeneuve, Daniel L.

AU - Zhang, Jiamin

AU - Wang, Zhihao

AU - Wei, Si

AU - Yu, Hongxia

AU - Zhang, Xiaowei

N1 - Funding Information: For support, we thank National Natural Science Foundation of China (grant nos. 21322704 and 21677072) and Jiangsu Province Funds for Distinguished Young Scientists (BK20130015). X.Z. was supported by the Fundamental Research Funds for the Central Universities and the Collaborative Innovation Center for Regional Environmental Quality. Although one of the authors is an employee of the United States Environmental Protection Agency (U.S. EPA), the contents of this manuscript neither represent nor necessarily reflect U.S. EPA policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. Publisher Copyright: © 2016 American Chemical Society.

PY - 2016/11/15

Y1 - 2016/11/15

N2 - Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are analogs of PBDEs with hundreds of possible structures and are frequently detected in the environment. However, the in vivo evidence on the toxicity of OH-PBDEs is still very limited. Here, the developmental toxicity of 6-OH-BDE47, a predominant congener of OH-PBDEs detected in the environment, in chicken embryos was assessed using a toxicogenomic approach. Fertilized chicken eggs were dosed via in ovo administration of 0.006 to 0.474 nmol 6-OH-BDE47/g egg followed by 18 days of incubation. Significant embryo lethality (LD50 = 1.940 nmol/g egg) and increased hepatic somatic index (HSI) were caused by 6-OH-BDE47 exposure. The functional enrichment of differentially expressed genes (DEGs) was associated with oxidative phosphorylation, generation of precursor metabolites and energy, and electron transport chains, which suggest that 6- OH-BDE47 exposure may disrupt the embryo development by altering the function of energy production in mitochondria. Moreover, aryl hydrocarbon receptor (AhR)- mediated responses including up-regulation of CYP1A4 were observed in the livers of embryos exposed to 6-OH-BDE47. Overall, this study confirmed the embryo lethality by 6-OH-BDE47 and further improved the mechanistic understanding of OH-PBDEs-caused toxicity. Ecological risk assessment via application of both no-observed-effect level (NOEL) and the sensitive NOTEL (transcriptional NOEL) suggested that OH-PBDEs might cause ecological risk to wild birds.

AB - Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are analogs of PBDEs with hundreds of possible structures and are frequently detected in the environment. However, the in vivo evidence on the toxicity of OH-PBDEs is still very limited. Here, the developmental toxicity of 6-OH-BDE47, a predominant congener of OH-PBDEs detected in the environment, in chicken embryos was assessed using a toxicogenomic approach. Fertilized chicken eggs were dosed via in ovo administration of 0.006 to 0.474 nmol 6-OH-BDE47/g egg followed by 18 days of incubation. Significant embryo lethality (LD50 = 1.940 nmol/g egg) and increased hepatic somatic index (HSI) were caused by 6-OH-BDE47 exposure. The functional enrichment of differentially expressed genes (DEGs) was associated with oxidative phosphorylation, generation of precursor metabolites and energy, and electron transport chains, which suggest that 6- OH-BDE47 exposure may disrupt the embryo development by altering the function of energy production in mitochondria. Moreover, aryl hydrocarbon receptor (AhR)- mediated responses including up-regulation of CYP1A4 were observed in the livers of embryos exposed to 6-OH-BDE47. Overall, this study confirmed the embryo lethality by 6-OH-BDE47 and further improved the mechanistic understanding of OH-PBDEs-caused toxicity. Ecological risk assessment via application of both no-observed-effect level (NOEL) and the sensitive NOTEL (transcriptional NOEL) suggested that OH-PBDEs might cause ecological risk to wild birds.

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Toxicogenomic assessment of 6-OH-BDE47-induced developmental toxicity in chicken embryos

Abstract

Bibliographical note

ASJC Scopus subject areas

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