Tooth slice-based models for the study of human dental pulp angiogenesis

SB Gonçalves; Z Dong; CM Bramante; GR Holland; Anthony Smith; Jacques Nor

doi:10.1016/j.joen.2007.03.012

Tooth slice-based models for the study of human dental pulp angiogenesis

SB Gonçalves, Z Dong, CM Bramante, GR Holland, Anthony Smith, Jacques Nor

Dentistry

Research output: Contribution to journal › Article

49 Citations (Scopus)

Abstract

Treatment of avulsed young permanent teeth aims to revascularize the dental pulp. The study of therapeutic strategies for avulsed teeth has been hindered by the scarcity of experimental models. The purpose of this work is to characterize two model systems to study dental pulp revascularization. Tooth slices from human third molars were prepared with a sterile diamond saw. The tooth slices were cultured in vitro for up to 7 days. Immunohistochemical staining with Factor VIII showed an increase in microvascular density in pulps treated with 50 ng/mL rhVEGF(165) as compared with untreated controls (p <0.05). Alternatively, tooth slices were prepared and immediately implanted subcutaneously in immunodeficient mice. Pulp vitality and vascularization were confirmed by histological analysis and terminal deoxynucleotide transferase dUTP nick end labeling assays 7 days after implantation. The models presented here may be valuable in the assessment of angiogenesis-based therapeutic strategies for the dental pulp.

Original language	English
Pages (from-to)	811-4
Number of pages	4
Journal	Journal of Endodontics
Volume	33
Issue number	7
DOIs	https://doi.org/10.1016/j.joen.2007.03.012
Publication status	Published - 1 Jul 2007

Access to Document

10.1016/j.joen.2007.03.012

Cite this

@article{3b9f73339238423a87a5025b05c4206c,

title = "Tooth slice-based models for the study of human dental pulp angiogenesis",

abstract = "Treatment of avulsed young permanent teeth aims to revascularize the dental pulp. The study of therapeutic strategies for avulsed teeth has been hindered by the scarcity of experimental models. The purpose of this work is to characterize two model systems to study dental pulp revascularization. Tooth slices from human third molars were prepared with a sterile diamond saw. The tooth slices were cultured in vitro for up to 7 days. Immunohistochemical staining with Factor VIII showed an increase in microvascular density in pulps treated with 50 ng/mL rhVEGF(165) as compared with untreated controls (p <0.05). Alternatively, tooth slices were prepared and immediately implanted subcutaneously in immunodeficient mice. Pulp vitality and vascularization were confirmed by histological analysis and terminal deoxynucleotide transferase dUTP nick end labeling assays 7 days after implantation. The models presented here may be valuable in the assessment of angiogenesis-based therapeutic strategies for the dental pulp.",

author = "SB Gon{\c c}alves and Z Dong and CM Bramante and GR Holland and Anthony Smith and Jacques Nor",

year = "2007",

month = jul,

day = "1",

doi = "10.1016/j.joen.2007.03.012",

language = "English",

volume = "33",

pages = "811--4",

journal = "Journal of Endodontics",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Tooth slice-based models for the study of human dental pulp angiogenesis

AU - Gonçalves, SB

AU - Dong, Z

AU - Bramante, CM

AU - Holland, GR

AU - Smith, Anthony

AU - Nor, Jacques

PY - 2007/7/1

Y1 - 2007/7/1

N2 - Treatment of avulsed young permanent teeth aims to revascularize the dental pulp. The study of therapeutic strategies for avulsed teeth has been hindered by the scarcity of experimental models. The purpose of this work is to characterize two model systems to study dental pulp revascularization. Tooth slices from human third molars were prepared with a sterile diamond saw. The tooth slices were cultured in vitro for up to 7 days. Immunohistochemical staining with Factor VIII showed an increase in microvascular density in pulps treated with 50 ng/mL rhVEGF(165) as compared with untreated controls (p <0.05). Alternatively, tooth slices were prepared and immediately implanted subcutaneously in immunodeficient mice. Pulp vitality and vascularization were confirmed by histological analysis and terminal deoxynucleotide transferase dUTP nick end labeling assays 7 days after implantation. The models presented here may be valuable in the assessment of angiogenesis-based therapeutic strategies for the dental pulp.

AB - Treatment of avulsed young permanent teeth aims to revascularize the dental pulp. The study of therapeutic strategies for avulsed teeth has been hindered by the scarcity of experimental models. The purpose of this work is to characterize two model systems to study dental pulp revascularization. Tooth slices from human third molars were prepared with a sterile diamond saw. The tooth slices were cultured in vitro for up to 7 days. Immunohistochemical staining with Factor VIII showed an increase in microvascular density in pulps treated with 50 ng/mL rhVEGF(165) as compared with untreated controls (p <0.05). Alternatively, tooth slices were prepared and immediately implanted subcutaneously in immunodeficient mice. Pulp vitality and vascularization were confirmed by histological analysis and terminal deoxynucleotide transferase dUTP nick end labeling assays 7 days after implantation. The models presented here may be valuable in the assessment of angiogenesis-based therapeutic strategies for the dental pulp.

U2 - 10.1016/j.joen.2007.03.012

DO - 10.1016/j.joen.2007.03.012

M3 - Article

C2 - 17804317

VL - 33

SP - 811

EP - 814

JO - Journal of Endodontics

JF - Journal of Endodontics

IS - 7

ER -

Tooth slice-based models for the study of human dental pulp angiogenesis

Abstract

Access to Document

Fingerprint

Cite this