TY - JOUR
T1 - The contrasting activity of iodido versus chlorido ruthenium and osmium arene azo- and imino-pyridine anticancer complexes
T2 - Control of cell selectivity, cross-resistance, p53 dependence, and apoptosis pathway
AU - Romero-Canelón, Isolda
AU - Salassa, Luca
AU - Sadler, Peter J.
PY - 2013/2/14
Y1 - 2013/2/14
N2 - Organometallic half-sandwich complexes [M(p-cymene)(azo/imino-pyridine)X] + where M = RuII or OsII and X = Cl or I, exhibit potent antiproliferative activity toward a range of cancer cells. Not only are the iodido complexes more potent than the chlorido analogues, but they are not cross-resistant with the clinical platinum drugs cisplatin and oxaliplatin. They are also more selective for cancer cells versus normal cells (fibroblasts) and show high accumulation in cell membranes. They arrest cell growth in G1 phase in contrast to cisplatin (S phase) with a high incidence of late-stage apoptosis. The iodido complexes retain potency in p53 mutant colon cells. All complexes activate caspase 3. In general, antiproliferative activity is greatly enhanced by low levels of the glutathione synthase inhibitor l-buthionine sulfoxime. The work illustrates how subtle changes to the design of low-spin d6 metal complexes can lead to major changes in cellular metabolism and to potent complexes with novel mechanisms of anticancer activity.
AB - Organometallic half-sandwich complexes [M(p-cymene)(azo/imino-pyridine)X] + where M = RuII or OsII and X = Cl or I, exhibit potent antiproliferative activity toward a range of cancer cells. Not only are the iodido complexes more potent than the chlorido analogues, but they are not cross-resistant with the clinical platinum drugs cisplatin and oxaliplatin. They are also more selective for cancer cells versus normal cells (fibroblasts) and show high accumulation in cell membranes. They arrest cell growth in G1 phase in contrast to cisplatin (S phase) with a high incidence of late-stage apoptosis. The iodido complexes retain potency in p53 mutant colon cells. All complexes activate caspase 3. In general, antiproliferative activity is greatly enhanced by low levels of the glutathione synthase inhibitor l-buthionine sulfoxime. The work illustrates how subtle changes to the design of low-spin d6 metal complexes can lead to major changes in cellular metabolism and to potent complexes with novel mechanisms of anticancer activity.
UR - http://www.scopus.com/inward/record.url?scp=84873919075&partnerID=8YFLogxK
U2 - 10.1021/jm3017442
DO - 10.1021/jm3017442
M3 - Article
C2 - 23368735
AN - SCOPUS:84873919075
SN - 0022-2623
VL - 56
SP - 1291
EP - 1300
JO - Journal of Medicinal Chemistry
JF - Journal of Medicinal Chemistry
IS - 3
ER -