tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Daniel J Nieves, Geva Hilzenrat, Jason Tran, Zhengmin Yang, Hugh H MacRae, Matthew A B Baker, J Justin Gooding, Katharina Gaus

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
136 Downloads (Pure)

Abstract

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

Original languageEnglish
Article number191268
JournalRoyal Society Open Science
Volume6
Issue number12
DOIs
Publication statusPublished - 11 Dec 2019

Keywords

  • protein tags
  • monovalent
  • covalent
  • DNA-PAINT imaging
  • superresolution

Fingerprint

Dive into the research topics of 'tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging'. Together they form a unique fingerprint.

Cite this